Rickettsial Panel (RMSF, Typhus), IgG
Laboratory Fee Schedule
Procedure #: MCR0317A
CPT: 87040, 87070, 87077
Synonym(s): | Rickettsia Rickettsii and Rickettsia Typhi IgG Antibody Detection by Indirect Immunofluorescence Assay (IFA) |
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Requisition Form | G-2A |
Test Description | Standard IFA using a two-stage sandwich immunofluorescence technique for the detection of IgG antibodies to Rocky Mountain Spotted Fever and/or Typhus Fever antigens. |
Pre-Approval Needed | N/A |
Supplemental Information Required | N/A |
Supplemental Form(s) | N/A |
Performed on Specimens from (sources) | Human |
Sample/Specimen Type for Testing | Serum is preferred, plasma is accepted. |
Minimum Volume/Size Required | 1.0 mL |
Storage/Preservation Prior to Shipping | Specimens may be stored at 2°C to 8°C if testing will occur within 2 days; otherwise store frozen at -15°C or colder |
Transport Medium | N/A |
Specimen Labeling | At least 2 patient specific identifiers: First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. Medical Record number); must match information on submission form (G-2A). |
Shipping and Specimen Handling Requirements |
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Method | Immunofluorescence Assay for the detection of antibodies |
Turn-around Time | 5 working days. |
Interferences/Limitations | Gross hemolysis, lipemia, bacterial contamination |
Common Causes for Rejection | Insufficient quantity, unacceptable specimen type or source, improper shipping conditions, expired media or collection container, discrepancies between specimen label and submission form, and/or incomplete or missing submission form. |
Additional Information | In order to confirm an infection was recent, a convalescent phase sample should be submitted. The most convincing evidence of recent rickettsial disease is a fourfold rise in antibody titer between an acute phase serum and a convalescent phase serum. |