| Requisition Form |
G-2A |
| Test Description |
Qualitative Determination of IgG Antibodies against Schistosoma mansoni by Enzyme Linked Immunosorbent Assay (ELISA) |
| Pre-Approval Needed |
N/A |
| Supplemental Information Required |
N/A |
| Supplemental Form(s) |
N/A |
| Performed on Specimens from (sources) |
Human |
| Sample/Specimen Type for Testing |
Serum |
| Minimum Volume/Size Required |
500 µL |
| Storage/Preservation Prior to Shipping |
- Store serum cold (2°C to 8°C) if testing will occur within 5 days of collection.
- Store serum frozen (– 20°C) if testing will occur after 5 days of collection.
|
| Transport Medium |
N/A |
| Specimen Labeling |
- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form.
|
| Shipping and Specimen Handling Requirements |
Ship specimens:
- Ship all specimens overnight with enough cold packs or dry ice to maintain specimens at proper temperature until arrival at DSHS Laboratory.
- Ship Monday-Thursday; avoid weekend deliveries.
- Ship cold specimens overnight on ice packs if arriving at laboratory within 5 days from collection.
- Ship frozen specimens overnight on dry ice or on ice packs.
- Specimens must be received cold/frozen by the laboratory.
- according to Dangerous Good Regulations, IATA, and/or CFR 49
- using universal precautions for infectious agents
- triple-contained in accordance with federal shipping regulations for infectious agents
- as Biological Substance Category B
- Additional forms & resources
|
| Method |
Enzyme-Linked Immunosorbent Assay (ELISA) |
| Turn-around Time |
6 working days |
| Interferences/Limitations |
- Interference observed with hemolytic, lipemic and icteric samples.
- Bacterial contamination, heat-inactivation, or repeated freeze-thaw cycles may affect results.
|
| Common Causes for Rejection |
- Insufficient quantity,
- unacceptable specimen type or source,
- improper shipping conditions,
- expired media or collection container,
- discrepancies between specimen label and submission form, and/or
- incomplete or missing submission form
|
| Additional Information |
Diagnosis of an infectious disease should not be established based on a single test result. Diagnosis of schistosomiasis should be based on clinical history, signs, and symptoms in conjunction with diagnostic results. |