BurkholderiaPCR

PCR: Burkholderia mallei/pseudomallei

Laboratory Fee Schedule

Procedure #:  BTA0026

CPT:  87798

PCR: Burkholderia mallei/pseudomallei

Synonym(s):

Melioidosis or Burkholdeia pseudomallei, glanders or Burkholderia mallei PCR

Requisition Form

G-27A

Test Description

Detection of Burkholderia mallei/pseudomallei DNA by PCR

Pre-Approval Needed

Must notify BioThreat Team prior to sample submission: (512) 689-5537

Supplemental Information Required

N/A

Supplemental Form(s)

N/A

Performed on Specimens from (sources)

  • Non-Human (Bacterial)
  • Human
  • Environmental 

Sample/Specimen Type for Testing

  • Culture isolates
  • Whole blood
  • Serum
  • Environmental specimens

Minimum Volume/Size Required

Culture isolates

  • 1 plate or slant

Clinical samples

  • 1 mL

Storage/Preservation Prior to Shipping

 Culture isolates

  • Ambient

Clinical or environmental samples

  • 2-8 °C (cold)

Transport Medium

N/A

Specimen Labeling

Human

  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match submission form.

Non-Human or Environmental

  • Sample identification must match submission form.

Shipping and Specimen Handling Requirements

  • Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
  • Handled as infectious agent using universal precautions. 
  • Triple contained in accordance with federal shipping regulations for infectious agents. 
  • See also Emergency Preparedness resources online..

Notify BioThreat Team prior to sample submission: (512) 689-5537

Method

PCR

Turn-around Time

Preliminary result 1 day after receipt, final result 1- 21 days

Interferences/Limitations

Improper storage or shipping temperatures may limit organism recovery.

Common Causes for Rejection

  • Insufficient quantity
  • Unacceptable specimen type or source
  • Improper shipping conditions
  • Expired media or collection container
  • Discrepancies between specimen label and submission form
  • Incomplete or missing submission form

Additional Information

May be paired with Definitive ID: Brucella species

Last updated October 21, 2022