|
Synonym(s):
|
Serotyping
|
|
Requisition Form
|
G-2B for reportable organisms
|
|
Test Description
|
Serotyping
of Shiga-toxin positive Escherichia coli from reference culture or isolates
from clinical samples
|
|
Pre-Approval Needed
|
N/A
|
|
Supplemental Information Required
|
N/A
|
|
Supplemental Form(s)
|
N/A
|
|
Performed on Specimens from (sources)
|
Human
|
|
Sample/Specimen Type for Testing
|
Pure
isolate, shiga-toxin positive clinical sample
|
|
Minimum Volume/Size Required
|
At least
one viable colony
|
|
Storage/Preservation Prior to Shipping
|
Refrigerated
|
|
Transport Medium
|
Appropriate
medium that supports growth
|
|
Specimen Labeling
|
- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form.
|
|
Shipping and Specimen Handling Requirements
|
- Ship according to Dangerous Goods Regulations,
IATA, and/or CFR 49.
- Ship specimens overnight
Monday-Thursday, to avoid weekend deliveries.
|
|
Method
|
Bacterial culture
|
|
Turn-around Time
|
5-21 days
|
|
Interferences/Limitations
|
-
Material must be viable.
- Improper
storage or shipping temperatures limit organism recovery.
|
Common Causes for Rejection
|
-
Insufficient quantity
- Unacceptable
specimen type or source
- Improper shipping conditions
- Expired media or
collection container
- Discrepancies between specimen label and submission
form
- Incomplete or missing submission form.
|
|
Additional Information
|
- Serotyping
is performed from Shiga-toxin positive specimens only.
- Serotyping
is available for the 7 most common serotypes.
- Specimens that are non-reactive with these serotypes will be submitted
to the CDC for further testing.
|