Influenza SARS-CoV-2 (COVID-19)


Influenza SARS-CoV-2 (COVID-19)

Multiplex Real-Time RT-PCR 

Laboratory Fee Schedule

Procedure #: MCN0312A

Multiplex Real-Time RT-PCR Influenza SARS-CoV-2 (COVID-19)
Synonym(s): Influenza Surveillance, SARS-CoV-2, COVID-19
Requisition FormG-2V
Test DescriptionTesting of samples for the detection and differentiation of SARS-CoV-2, influenza A virus, and/or influenza B virus nucleic acid in upper and lower respiratory specimens from individuals suspected of respiratory viral infection consistent with COVID-19.
Pre-Approval NeededAll specimens for COVID-19 testing require preapproval. Contact your Local Health Department or DSHS Public Health Region to ensure patient meets the criteria for testing and to obtain approval to test. DO NOT ship specimens prior to receiving this approval.
Supplemental Information RequiredN/A
Supplemental Form(s)N/A
Performed on Specimens from (sources) Human
Sample/Specimen Type for Testing
  • Nasopharyngeal swabs
  • Oropharyngeal (throat) swabs
  • Nasal swabs
  • Sputum
  • Lower respiratory tract aspirates
  • Bronchoalveolar lavage
  • Nasopharyngeal wash/aspirate
  • Nasal aspirate
Minimum Volume/Size Required 2-3 mL 
Storage/Preservation Prior to Shipping Refrigerate at 2-8°C, or freeze at -70°C or below
Transport Medium 
  • Universal transport media (UTM)
  • Viral transport media (VTM)
Specimen Labeling 
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number).
  • Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements 

Ship samples:

  • According to Dangerous Good Regulations, IATA, and/or CFR 49.
  • Handling as infectious agent using universal precautions.
  • Triple-contained in accordance with federal shipping regulations for infectious agents
  • If specimens are expected to arrive at the lab within 72 hours of collection, refrigerate at 2-8°C and ship overnight on ice packs.
  • If specimens are expected to arrive at the lab after 72 hours of collection, freeze at -70°C and ship overnight on dry ice.
Turn-around Time2 business days 
Interferences/Limitations 
  • False negative may result if specimen is improperly collected, transported, or handled
  • Detection of viral RNA does not imply that the corresponding virus is infectious or is the causative agent for clinical symptoms.
  • A negative result does not preclude infection.
Common Causes for Rejection 
  • Information on the submission form and sample do not match.
  • Failure to put two forms of identification on sample.
  • Specimen received cold greater than 72 hours after collection.
  • Specimen received ambient.
  • Specimen leaked in transit.
  • Submission of incorrect sample/specimen type.
  • Submission of incorrect or expired transport medium.
Additional Information Please mark the Influenza Surveillance {Influenza PCR} and COVID-19 (SARS-CoV-2) PCR boxes on the G-2V form.
Note: By checking the Influenza Surveillance or COVID-19 PCR test request box, submitters authorize DSHS to test for Flu and/or COVID as resources allow 


Last updated August 18, 2022