||The quantitation of HDL cholesterol in human serum is inversely associated with the risk of coronary heart disease.
|Supplemental Information Required
|Performed on Specimens from (sources)
|Sample/Specimen Type for Testing
- Preferred: Serum from a red top tube transferred to a transport tube
- Acceptable: Serum from a centrifuged gold top serum separator tube.
|Minimum Volume/Size Required
|Storage/Preservation Prior to Shipping
- Collect blood in red top or gold top tube.
- Gently invert the tube with the blood specimen five times immediately after collection.
- Allow to clot 30 minutes from collection.
- Centrifuge gold top tube to separate serum from red blood cells.
- For red top tube: Immediately transfer serum into a transport tube.
- For gold top tube: Immediately transfer serum into a transport tube if serum will be frozen.*
- Store serum cold if testing will occur within 7 days of collection.
- Store serum frozen if testing will occur after 7 days of collection.
NOTE: * Gold top serum separator tube cannot be frozen. Serum must be transferred to a transport tube prior to freezing.
- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number
- Identifiers on specimen must exactly match submission form.
|Shipping and Specimen Handling Requirements
- Ship all specimens overnight with enough cold packs or dry ice to maintain specimens at proper temperature until arrival at DSHS Laboratory.
- Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
- Handle as infectious agent using universal precautions
- Ship triple-contained in accordance with federal shipping regulations for infectious agents
- Ship Monday-Thursday; avoid Friday and weekend deliveries
- Ship cold specimens overnight on ice packs if arriving at laboratory within 7 days from collection.
- Ship frozen specimens overnight on dry ice or on ice packs.
- Specimens must be received cold/frozen by the laboratory.
- Consider freezing serum transport tubes prior to shipping during high temperature months.
||4-5 working days after receipt of specimen
||Lipemia, turbidity, icterus, hemolysis, triglyceride value >1000 mg/dL.
|Common Causes for Rejection
- Two patient-specific identifiers not met
- Specimen received at ambient temperature due to inadequate/no ice packs.
- Specimen unsuitable for testing
- Specimen was received at improper temperature
- No specimen included with submission form.
- Submission form not included with specimen.
- Specimen tube broken/leaked in transit.
- Demographic information is missing or incorrect.
- Specimen identification is missing or incorrect.
- Whole blood was received.
- Specimen is collected in an expired blood collection tube.
- No test specified.
- Red top tubes have not had serum poured over to a transport tube.
- Frozen gold top tubes.
- Children/adolescents (<20 years): >45 mg/dL
- Young adults (20-24 years): >45 mg/dL
- Adults: 40 - 59 mg/dL