Mycobacterium tuberculosis complex, AFB Agar Proportion Drugs, Kanamycin Susceptibility

Laboratory Fee Schedule
Procedure: MAD0066A
CPT: 87190

Synonym(s):M. tuberculosis complex drug susceptibility test (DST), KAN DST, KM DST
Requisition FormG-MYCO
Test DescriptionSusceptibility test to Kanamycin by agar proportion method.
Pre-Approval NeededN/A
Supplemental Information RequiredN/A
Supplemental Form(s)N/A  
Performed on Specimens from (sources)Pure isolate from human sources
Sample/Specimen Type for TestingM. tuberculosis complex pure isolate in liquid or on solid culture medium
Minimum Volume/Size Required
  • Liquid culture - 2.0 mL
  • Solid culture - Visible growth, more than a single colony
Storage/Preservation Prior to ShippingAmbient
Transport MediumN/A
Specimen Labeling
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match those on the submission form.
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49
  • Triple-contain in accordance with federal shipping regulations for UN2814 Category A, Infectious substance, affecting humans.
  • Anyone who handles Category A substances is required by law to be trained in handling and shipping Category A, Infectious Substances.
    • Untrained personnel should not handle or ship M. tuberculosis isolates. 
  • Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
     
MethodAgar proportion susceptibility test interpreted as either susceptible or resistant to a specific drug at critical concentration recommended by CLSI
Turn-around Time14 to 21 days
Interferences/LimitationsTest can only be performed on pure cultures of M. tuberculosis complex
Common Causes for Rejection
  • Non-viable isolates.
  • Cultures overgrown by contaminating organisms.
  • Isolate not M. tuberculosis complex.
Additional Information
  • Isolates submitted for this test will be identified by this laboratory. 
  • No fee will be charged for this identification unless identification is requested on the G-MYCO requisition form.
  • This laboratory will notify submitter of new drug resistance detected by this test.