Listeria species - Identification

Laboratory Fee Schedule
Procedure:  MAQ0111A
CPT: N/A    

Synonym(s): Listeria; Listeria species
Requisition Form G-22-(Food); G-23; G-21 Path-Pathogen; MSA-FORM 50-1
Test Description Food analysis method used to identify Listeria species (including Listeria monocytogenes) includes enrichment, rapid screen, isolation, and biochemical testing
Pre-Approval Needed Regulatory or IDCU agent
Supplemental Information Required N/A
Supplemental Form(s) N/A  
Performed on Specimens from (sources)
  • Food, Milk
  • Environmental 
Sample/Specimen Type for Testing
  • Food/milk samples
  • Environmental swabs
Minimum Volume/Size Required

100 grams (1/4 pound)

Storage/Preservation Prior to Shipping
  • Environmental swabs must be collected on appropriate swabs used for environmental testing (e.g., 3M™ Quick Swabs or Spongesicle type swabs or equivalent).
  • Place food sample in sterile zip-lock or whirlpack bag or other sterile plastic container. 
  • Store on ice.
Transport Medium N/A
Specimen Labeling Sample Identification must match submission form.
Shipping and Specimen Handling Requirements
  • Ship samples on Ice and/or Ice Packs.
  • Samples must be shipped for overnight delivery.
  • Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
Method
  • BAX® Real-Time PCR Screen
  • VIDAS® ELFA Screen
  • FDA Bacteriological Analytical Manual (BAM)- Chapter 10
Turn-around Time 14 Days
Interferences/Limitations
  • The sample should represent the outer surface as well as the interior of the food.
  • Environmental swabs must be tested within 48 hours of collection.
  • Milk Samples must be tested within 60 hours of collection
Common Causes for Rejection
  • Not collected by a Registered Sanitarian
  • Insufficient quantity
  • Improperly stored
  • Failure to meet shipping guidelines
     
Additional Information N/A