Laboratory Fee Schedule
Procedure: MMV0104A
CPT: N/A
| Synonym(s): | WNV, West Nile virus, SLEV, St. Louis encephalitis virus, EEEV, Eastern equine encephalitis virus, flavivirus, alphavirus, arbovirus, mosquito, reverse transcriptase-polymerase chain reaction, virus isolation, cell culture, indirect fluorescent antibody test, IFA |
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| Requisition Form | G-14 |
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| Test Description | Real-time RT-PCR multiplex assay for detection of West Nile virus, St. Louis encephalitis virus, and Eastern equine encephalitis virus in mosquito samples. |
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| Pre-Approval Needed | Contact the Arbovirus/Entomology Team at ArbovirusEntomologyTeam@dshs.texas.gov to obtain G-14 specimen submission forms and the required shipping materials for submitting mosquito samples to the DSHS Laboratory. |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | |
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| Sample/Specimen Type for Testing | Live, adult mosquitoes |
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| Minimum Volume/Size Required | 1 (one) mosquito |
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| Storage/Preservation Prior to Shipping | - Use a handheld aspirator to transfer up to 100 live mosquitoes from each trap collection to a DSHS Lab-provided plastic shipping carton.
- Submit only one carton per trap.
- Store at room temperature until shipment.
- Do not freeze mosquitoes.
- Ship mosquitoes as soon as possible after collection.
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| Transport Medium | N/A |
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| Specimen Labeling | - Each shipping carton must be submitted with a completed G-14 submission form.
- Attach submission form to carton using a rubber band.
- Ensure collection data is provided on submission form.
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| Shipping and Specimen Handling Requirements | - Ship mosquitoes cool to the Lab using the insulated boxes provided by the Arbovirus/Entomology Laboratory.
- Include provided coolant bottle in the shipment.
- Fill bottle with water to the line, tighten cap, and freeze. Place frozen bottle in plastic bag (provided) and wrap with damp newspaper or paper towels.
- Add several sheets of moistened paper towels or newspaper to the inner box to keep humidity high. Avoid saturating the paper. Excess water in cartons will damage mosquitoes.
- Tape the shipping box closed and affix required labels to exterior of the box (boxes are reused).
- If replacement or additional shipping supplies are needed, contact the Arbovirus/Entomology Team by emailing ArbovirusEntomologyTeam@dshs.texas.gov.
- Ship for delivery to Lab during business hours only (Monday to Friday 8 a.m. – 5 p.m.). Do not ship on Fridays.
- Shipping overnight or next day is preferred.
- Additional arbovirus field surveillance techniques are available online here.
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| Method | Real-time RT-PCR multiplex assay |
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| Turn-around Time | 5 days |
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| Interferences/Limitations | N/A |
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| Common Causes for Rejection | - Incorrect storage conditions of mosquitoes.
- Species identification of mosquitoes received in poor condition may not be possible.
- Mosquitoes that cannot be identified to the species level will not be tested.
- Non-vector species submitted.
- Only vector species are tested.
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| Additional Information | - Additional arbovirus testing using cell culture screening methods is conducted on 20 percent of submitted mosquito trap collections. This testing cannot be ordered by submitters.
- Arbovirus identification using an indirect fluorescent antibody test is conducted as a reflex test when cytopathic effects are detected during cell culture screening. This testing cannot be ordered by submitters.
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