| Synonym(s): | Cryptosporidium spp.; Subtyping; Sanger Sequencing; Cryptosporidium; Crypto; Cryptosporidiosis; Nested PCR |
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| Requisition Form | G2-B |
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| Test Description | Subtyping of Cryptosporidium spp. by nested PCR and Sanger sequencing. |
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| Pre-Approval Needed | N/A |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Human |
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| Sample/Specimen Type for Testing | Stool |
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| Minimum Volume/Size Required | At least 500 µL |
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| Storage/Preservation Prior to Shipping | - Raw stool and unfixed stool should be stored and shipped cold between 2°C–8°C.
- Fixed stool may be shipped at ambient temperature.
- Avoid specimen collection kits containing formalin as it interferes with testing.
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| Transport Medium | - Raw stool, or stool in Cary Blair medium
- Stool treated with non-formalin and PCR compatible fixatives
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| Specimen Labeling | - At least two patient identifiers are required on specimen (e.g., patient full name, date of birth, medical record number).
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements | - Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Handle as infectious agent using universal precautions.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Ship raw stool specimens cold and overnight Monday-Thursday to avoid weekend deliveries.
- Additional DSHS Laboratory specimen labeling guidelines online here.
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| Method | Nested PCR and Sanger sequencing |
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| Turn-around Time | 14 working days |
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| Interferences/Limitations | Only samples positive for Cryptosporidium can be subtyped. |
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| Common Causes for Rejection | - inadequate specimen volume
- improperly stored specimen
- raw stool specimens not stored cold
- specimen dried out
- specimen over two years old
- failure to meet shipping/transportation guidelines
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| Additional Information | N/A |
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