Enterohaemorrhagic Escherichia Coli (EHEC), Shiga- like Toxin Assay

Laboratory Fee Schedule
Procedure #: MAA0047A
CPT: 87427

Synonym(s):	Shiga-like toxin, STX-1 STX-2, STEC, EHEC, E. coli toxin
Requisition Form	G-2B
Test Description	Escherichia coli shiga toxin assay
Pre-Approval Needed	N/A
Supplemental Information Required	N/A
Supplemental Form(s)	N/A
Performed on Specimens from (sources)	Human
Sample/Specimen Type for Testing	Pure culture E. coli; Clinical stool
Minimum Volume/Size Required	Pure culture: At least one viable colony Clinical sample: 500 µl
Storage/Preservation Prior to Shipping	Pure Culture: Refrigerated Broth/liquid transport Refrigerated: Received < 7 days Frozen @-70C: Received > 7 days
Transport Medium	Media supporting growth of isolate Clinical sample in GN broth or transport media
Specimen Labeling	Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number) Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements	Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49. Ship specimens overnight Monday-Thursday, to avoid weekend deliveries.
Method	Enzyme Immunoassay
Turn-around Time	3-21 days
Interferences/Limitations	Material must be viable Improper storage or shipping temperatures limit organism recovery.
Common Causes for Rejection	Insufficient quantity Unacceptable specimen type or source Improper shipping conditions Expired media or collection container Discrepancies between specimen label and submission form Incomplete or missing submission form
Additional Information	Shiga toxin-producing Escherichia coli infection (all E.coli O157:H7 isolates and any E.coli isolates or specimens in which Shiga toxin activity has been demonstrated (STX1, STX2) are required to be submitted per Texas Administrative code (TAC) chapter 97: §97.3(a)(4), §97.4(a)(6), and §97.5(a)(2)(C) https://www.dshs.state.tx.us/idcu/investigation/conditions/