Escherichia coli Shiga Toxin Virulence Factor Detection- PCR

Laboratory Fee Schedule
Procedure #: MMB0004A
CPT: 87150

Synonym(s):	E. coli, PCR
Requisition Form	G-2B
Test Description	Real time polymerase chain reaction (PCR) for the detection of virulence factors such as stx₁, stx₂, eaeA, and ipaH
Pre-Approval Needed	N/A
Supplemental Information Required	N/A
Supplemental Form(s)	N/A
Performed on Specimens from (sources)	Human Food Environmental
Sample/Specimen Type for Testing	Pure isolates are preferred
Minimum Volume/Size Required	N/A
Storage/Preservation Prior to Shipping	N/A. Plated or broth pure isolates are referred directly from the DSHS Clinical Bacteriology Team.
Transport Medium	N/A
Specimen Labeling	Human At least two patient identifiers are required on specimen (e.g., patient full name, date of birth, medical record number). Three patient identifiers are preferred. Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.) Environmental or Food Sample Sample identification must match submission form.
Shipping and Specimen Handling Requirements	Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49. Category B Biological Substance, UN3373 shipping requirements apply. Handle as infectious agent using universal precautions. Triple-contain in accordance with federal shipping regulations. Submitters are responsible for packaging and shipping specimens according to regulatory requirements. Ship specimens overnight Monday-Thursday to avoid weekend deliveries. Additional DSHS Laboratory specimen labeling guidelines online here.
Method	Real-time PCR
Turn-around Time	7 business days from receipt. If isolate is sent to CDC for further study, turnaround time may be longer.
Interferences/Limitations	N/A
Common Causes for Rejection	insufficient specimen growth mixed/impure isolate failure to meet submission/shipping guidelines
Additional Information	May be paired with bacteriological culture