Laboratory Fee Schedule
Procedure: MIR00001A
CPT: N/A
| Synonym(s): | IR testing, bottle bioassay, mosquito, pyrethroid, organophosphate, Culex, Aedes |
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| Requisition Form | G-14 |
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| Test Description | - Mosquitoes are exposed to the active ingredient from the insecticide under evaluation.
- Mortality rates are documented at different timepoints to determine resistance or susceptibility to the active ingredient.
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| Pre-Approval Needed | Yes. Contact the Arbovirus/Entomology Team at ArbovirusEntomologyTeam@dshs.texas.gov to obtain G-14 specimen submission forms and the required shipping materials for submitting mosquito samples for insecticide resistance testing. Pre-approval required before submitting samples. |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | |
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| Sample/Specimen Type for Testing | - Live, adult Culex spp. mosquitoes
- Eggs of Aedes spp.
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| Minimum Volume/Size Required | Approximately 100 to 125 individual mosquitoes are required for testing |
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| Storage/Preservation Prior to Shipping | - Shipping materials for adult mosquito submissions are provided by the Arbovirus/Entomology Laboratory.
- For Culex species submissions, use a handheld aspirator to transfer live mosquitoes from each trap collection to a DSHS-provided plastic shipping carton.
- Do not freeze mosquitoes.
- Ship mosquitoes as soon as possible after collection.
- For Aedes species submissions, place each egg liner collection in a separate sealable plastic bag.
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| Transport Medium | N/A |
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| Specimen Labeling | - Each shipping carton or egg liner must have a G-14 submission form filled out and attached.
- Write “Insecticide Resistance Testing” in the comments section in Section 2 of G-14 form.
- Ensure collection data is provided on submission form.
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| Shipping and Specimen Handling Requirements | - For Culex species submissions, ship mosquitoes to the Lab using the insulated boxes provided by the Arbovirus/Entomology Laboratory.
- Include provided coolant bottle in the shipment.
- Fill bottle with water to the line, tighten cap, and freeze. Place frozen bottle in plastic bag (provided) and wrap with damp newspaper or paper towels.
- Add several sheets of moistened paper towels or damp newspaper to the inner box to keep humidity high. Avoid saturating the paper; excess water in cartons will damage mosquitoes.
- Close and tape the box with appropriate labels showing (boxes are reused).
- If replacement or additional shipping supplies are needed, contact the Arbovirus/Entomology Team by emailing ArbovirusEntomologyTeam@dshs.texas.gov.
- Ship for delivery to Lab during business hours only (Monday to Friday 8 a.m. – 5 p.m.). Do not ship on Fridays.
- Shipping overnight or next day is preferred.
- For Aedes species submissions, ship egg liners in a padded envelope.
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| Method | CDC Bottle Bioassay |
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| Turn-around Time | 28 days |
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| Interferences/Limitations | N/A |
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| Common Causes for Rejection | - Incorrect storage conditions of mosquitoes.
- Mosquitoes received in poor condition.
- Live mosquitoes are required for testing.
- Too few mosquitoes submitted for the bottle bioassay.
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| Additional Information | Contact the Arbovirus/Entomology Team at ArbovirusEntomologyTeam@dshs.texas.gov to participate in the insecticide resistance testing program. |
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