Synonym(s):
|
Bios,
Basic |
Requisition Form
|
G-MYCO
|
Test Description
|
Identification
of mycobacterial isolates referred to DSHS Laboratory by biochemical tests
|
Pre-Approval Needed
|
N/A
|
Supplemental Information Required
|
N/A
|
Supplemental Form(s)
|
N/A
|
Performed on Specimens from (sources)
|
Pure
isolate from human sources
|
Sample/Specimen Type for Testing
|
Mycobacterium
species pure isolate in liquid or on solid culture medium
|
Minimum Volume/Size Required
|
Liquid
culture - 2.0 mL Solid
culture - Single mature colony |
Storage/Preservation Prior to Shipping
|
Ambient
|
Transport Medium
|
N/A
|
Specimen Labeling
|
• Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number) • Identifiers on specimen must exactly match submission form. |
Shipping and Specimen Handling Requirements
|
• Ship according to Dangerous Goods Regulations,
IATA, and/or CFR 49 • Category
B shipping applies for unidentified isolates
|
Method
|
• Biochemical testing to detect the presence of
absence of an enzyme • Physiological testing
|
Turn-around Time
|
14
days
|
Interferences/Limitations
|
• Identification by biochemical tests is
performed in conjunction with the morphological characteristics of the
isolate and the HPLC pattern derived from cell wall analysis. • Biochemical testing can be affected by the age
of the culture. • Identification by biochemical testing is
limited and may not differentiate species without testing such as sequencing
or mass spectrometry.
|
Common Causes for Rejection
|
• Cultures
overgrown by mold • Cultures
broken in transit
|
Additional Information
|
Basic biochemical testing includes the most
common biochemical panel used in the identification of Mycobacterium species.
|