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Synonym(s):
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Bios,
Basic |
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Requisition Form
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G-MYCO
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Test Description
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Identification
of mycobacterial isolates referred to DSHS Laboratory by biochemical tests
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Pre-Approval Needed
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N/A
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Supplemental Information Required
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N/A
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Supplemental Form(s)
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N/A
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Performed on Specimens from (sources)
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Pure
isolate from human sources
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Sample/Specimen Type for Testing
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Mycobacterium
species pure isolate in liquid or on solid culture medium
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Minimum Volume/Size Required
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Liquid
culture - 2.0 mL Solid
culture - Single mature colony |
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Storage/Preservation Prior to Shipping
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Ambient
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Transport Medium
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N/A
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Specimen Labeling
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• Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
• Identifiers on specimen must exactly match submission form. |
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Shipping and Specimen Handling Requirements
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• Ship according to Dangerous Goods Regulations,
IATA, and/or CFR 49 • Category
B shipping applies for unidentified isolates
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Method
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• Biochemical testing to detect the presence of
absence of an enzyme • Physiological testing
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Turn-around Time
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14
days
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Interferences/Limitations
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• Identification by biochemical tests is
performed in conjunction with the morphological characteristics of the
isolate and the HPLC pattern derived from cell wall analysis. • Biochemical testing can be affected by the age
of the culture. • Identification by biochemical testing is
limited and may not differentiate species without testing such as sequencing
or mass spectrometry.
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| Common Causes for Rejection
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• Cultures
overgrown by mold • Cultures
broken in transit
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Additional Information
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Basic biochemical testing includes the most
common biochemical panel used in the identification of Mycobacterium species.
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