DS Microtiter Procedure for Brucella Agglutination Test

G-2A

The detection and quantitation of B. abortus IgG and IgM antibodies by the agglutination of safranin-O stained whole cells

N/A

N/A

N/A

Any

Serum

1.0 mL

• Blood should be allowed to clot at room temperature then centrifuged.

• Serum can be used if particulates are removed via filtration or by using lipid clearing reagents and centrifugation.

• Serum can be stored at 2°C to 8°C  if tested within two days or should be Frozen at ≤ -15°C or colder if not tested within 2 days.

N/A

• Two patient-specific identifiers required (e.g. patient full name, date of birth, Medical Record number)

• Identifiers on specimen must exactly match submission form.

• Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.

• Handle as infectious agent using universal precautions. 

• Triple contain in accordance with federal shipping regulations for infectious agents.
  Additional forms & resources

Antibody agglutination test

3 working days

Hemolytic, hyperlipemic and bacterial contamination can produce erroneous results in serologic tests.

• Insufficient quantity

• Unacceptable specimen type or source

• Improper shipping conditions

• Expired media or collection container

• Discrepancies between specimen label and submission form

• Incomplete or missing submission form.

This test can provide presumptive evidence of Brucellosis.  Results should be considered in combination with other clinical, laboratory and epidemiologic findings.