Laboratory Fee Schedule
Procedure: MZZ0135A
CPT: 86622
| Synonym(s): | Brucellosis, serum, serology, B. abortus, B. melitensis, B. suis, BMAT, Agglutination |
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| Requisition Form | G-2A |
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| Test Description | Detection and quantitation of Brucella antibodies using the Brucella Microagglutination Test (BMAT) |
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| Pre-Approval Needed | N/A |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Any |
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| Sample/Specimen Type for Testing | Serum |
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| Minimum Volume/Size Required | 1.0 mL |
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| Storage/Preservation Prior to Shipping | - Store serum cold at 2°C to 8°C for up to 48 hours after collection.
- Store serum frozen at -20°C or colder for storage longer than 48 hours.
- Transfer serum to transport tube before freezing.
- Never freeze serum in separator tubes.
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| Transport Medium | N/A |
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| Specimen Labeling | - At least two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three unique patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on G-2A submission form. (e.g. abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements | - Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Refrigerate at 2°C –8°C and ship overnight on frozen cold packs if specimens will arrive at the Laboratory within 48 hours of collection.
- Freeze at -20°C or colder, and ship overnight on dry ice if specimens will arrive at the Laboratory more than 48 hours after collection.
- Additional DSHS Laboratory specimen shipping guidelines online here.
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| Method | Microagglutination test |
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| Turn-around Time | 3 working days |
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| Interferences/Limitations | - Hemolytic, hyperlipemic and bacterial contamination can produce erroneous results in serologic tests.
- A negative result does not necessarily rule out brucellosis.
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| Common Causes for Rejection | - Insufficient quantity
- Unacceptable specimen type or source
- Improper shipping conditions
- Expired media or collection container
- Discrepancies between specimen label and submission form
- Incomplete or missing submission form.
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| Additional Information | - This test can provide presumptive evidence of brucellosis.
- Results should be considered in combination with other clinical, laboratory and epidemiologic findings.
- Antibodies to B. abortus, B. melitensis, and B. suis can be measured using this test.
- This test does not measure antibodies to B. canis or vaccine strain RB51
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