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Synonym(s):
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HDL Cholesterol
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Requisition Form
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G-1B, G-THSTEPS
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Test Description
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The quantitation of HDL
cholesterol in human serum is inversely associated with the risk of coronary heart
disease.
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Pre-Approval Needed
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N/A
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Supplemental Information Required
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N/A
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Supplemental Form(s)
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N/A
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Performed on Specimens from (sources)
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Human
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Sample/Specimen Type for Testing
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- Preferred: Serum from a
red top tube transferred to a transport tube
- Acceptable: Serum from a centrifuged gold top serum
separator tube.
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Minimum Volume/Size Required
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1mL serum
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Storage/Preservation Prior to Shipping
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- Collect blood in red top or gold top tube.
- Gently invert the tube with the blood specimen five times immediately after collection.
- Allow to clot 30 minutes from collection.
- Centrifuge gold top tube to separate serum from red blood cells.
- For red top tube: Immediately transfer serum into a transport tube.
- For gold top tube: Immediately transfer serum into a transport tube if serum will be frozen.*
- Store serum cold if testing will occur within 7 days of collection.
- Store serum frozen if testing will occur after 7 days of collection.
NOTE: * Gold top serum separator tube cannot be frozen. Serum must be transferred to a transport tube prior to freezing. |
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Transport Medium
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N/A
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Specimen Labeling
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Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number
- Identifiers on specimen must exactly match submission form.
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Shipping and Specimen Handling Requirements
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- Ship all specimens overnight with enough cold packs or dry ice to maintain specimens at proper temperature until arrival at DSHS Laboratory.
- Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
- Handle as infectious agent using universal precautions
- Ship triple-contained in accordance with federal shipping regulations for infectious agents
- Ship Monday-Thursday; avoid Friday and weekend deliveries
- Ship cold specimens overnight on ice packs if arriving at laboratory within 7 days from collection.
- Ship frozen specimens overnight on dry ice or on ice packs.
- Specimens must be received cold/frozen by the laboratory.
- Consider freezing serum transport tubes prior to shipping during high temperature months.
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Method
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Quantitative enzymatic
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Turn-around Time
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4-5 working days after receipt
of specimen
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Interferences/Limitations
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Lipemia, turbidity,
icterus, hemolysis, triglyceride value >1000 mg/dL.
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| Common Causes for Rejection
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- Two patient-specific identifiers not met
- Specimen received at ambient temperature due to inadequate/no ice packs.
- Specimen unsuitable for testing
- Specimen was received at improper temperature
- No specimen included with submission form.
- Submission form not included with specimen.
- Specimen tube broken/leaked in transit.
- Demographic information is missing or incorrect.
- Specimen identification is missing or incorrect.
- Whole blood was received.
- Specimen is collected in an expired blood collection tube.
- No test specified.
- Red top tubes have not had serum poured over to a transport tube.
- Frozen gold top tubes.
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Additional Information
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Reference Range:
- Children/adolescents
(<20 years): >45 mg/dL
- Young adults (20-24
years): >45 mg/dL
- Adults: 40 - 59 mg/dL
Contact:
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