| Synonym(s): | Total Hemoglobin, Hgb, Hb |
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| Requisition Form | G-1B, G-THSTEPS |
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| Test Description | The measurement of hemoglobin is used to detect anemia in whole blood specimens. |
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| Pre-Approval Needed | N/A |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Human |
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| Sample/Specimen Type for Testing | - Preferred: Whole blood in (BD) K2 EDTA lavender top vacuum tube (venous).
- Acceptable: Whole blood in (BD MAP) K2 EDTA lavender top tube (capillary for automation).
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| Minimum Volume/Size Required | Fill to manufacturer’s stated fill volume. Minimum of 1mL in a 2mL vacuum tube or 375µL minimum in a 500µL capillary for automation tube. |
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| Storage/Preservation Prior to Shipping | - Invert lavender top tubes at least 10 times immediately after collection to avoid clotting.
- Store at ambient or refrigerated temperature.
- Do not freeze.
- Avoid high temperature environments.
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| Transport Medium | N/A |
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| Specimen Labeling | - Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number
- Identifiers on specimen must exactly match submission form.
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| Shipping and Specimen Handling Requirements | - Specimens may be shipped at ambient or refrigerated temperature
- Specimens must be received by the laboratory within 14 days from collection.
- Do not freeze specimens.
- Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
- Handle as infectious agent using universal precautions
- Ship triple-contained in accordance with federal shipping regulations for infectious agents.
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| Method | Flow cytometry |
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| Turn-around Time | 4-5 working days after receipt of specimen |
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| Interferences/Limitations | Clotting, hemolysis, extremely elevated WBC’s, lipemia, abnormal proteins. |
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| Common Causes for Rejection | - Specimen is clotted.
- Specimen decomposed.
- Two patient-specific identifiers not met.
- Specimen is collected in an expired blood collection tube.
- Specimen is not mailed or shipped in a timely or proper manner.
- Demographic information is missing or incorrect.
- Specimen identification is missing or incorrect.
- Specimen tube broken/leaked in transit.
- Submission form not included with specimen.
- No specimen included with submission form.
- Specimen identification illegible
- Incorrect blood collection tube used.
- Specimen volume is insufficient or excessive.
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| Additional Information | Reference range: 11.0 - 16.0 g/dL Contact: |
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