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Synonym(s):
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Total Hemoglobin, Hgb
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Requisition Form
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G-1B, G-THSTEPS
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Test Description
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The measurement of
hemoglobin is used to detect anemia in whole blood specimens.
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Pre-Approval Needed
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N/A
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Supplemental Information Required
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N/A
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Supplemental Form(s)
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N/A
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Performed on Specimens from (sources)
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Human
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Sample/Specimen Type for Testing
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- Preferred: Whole blood in (BD) K2 EDTA
lavender top vacuum tube (venous).
- Acceptable: Whole blood in (BD MAP) K2 EDTA
lavender top tube (capillary for automation).
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Minimum Volume/Size Required
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Fill to manufacturer’s
stated fill volume. Minimum of 1mL in a 2mL
vacuum tube or 375µL minimum in a 500µL capillary for automation tube.
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Storage/Preservation Prior to Shipping
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- Invert lavender top tubes at least 10 times immediately
after collection to avoid clotting.
- Store at ambient or refrigerated temperature.
- Do not freeze.
- Avoid high temperature environments.
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Transport Medium
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N/A
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Specimen Labeling
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- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number
- Identifiers on specimen must exactly match submission form.
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Shipping and Specimen Handling Requirements
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Specimens may be shipped at ambient or refrigerated temperature
- Specimens must be received by the laboratory within 14 days from collection.
- Do not freeze specimens.
- Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
- Handle as infectious agent using universal precautions
- Ship triple-contained in accordance with federal shipping regulations for infectious agents.
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Method
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Flow cytometry
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Turn-around Time
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4-5 working days after
receipt of specimen
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Interferences/Limitations
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Clotting, hemolysis, extremely
elevated WBC’s, lipemia, abnormal proteins.
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| Common Causes for Rejection
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- Specimen is clotted.
- Two patient-specific identifiers not met.
- Specimen is collected in an expired blood collection tube.
- Specimen is not mailed or shipped in a timely or proper manner.
- Demographic information is missing or incorrect.
- Specimen identification is missing or incorrect.
- Specimen tube broken/leaked in transit.
- Submission form not included with specimen.
- No specimen included with submission form.
- Specimen identification illegible
- Incorrect blood collection tube used.
- Specimen volume is insufficient or excessive.
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Additional Information
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Reference range:
11.0 - 16.0 g/dL
Contact:
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