| Synonym(s): |
Total Hemoglobin, Hgb |
| Requisition Form |
G-1B, G-THSTEPS |
| Test Description |
The measurement of hemoglobin is used to detect anemia in whole blood specimens. |
| Pre-Approval Needed |
N/A |
| Supplemental Information Required |
N/A |
| Supplemental Form(s) |
N/A |
| Performed on Specimens from (sources) |
Human |
| Sample/Specimen Type for Testing |
- Preferred: Whole blood in (BD) K2 EDTA lavender top vacuum tube (venous).
- Acceptable: Whole blood in (BD MAP) K2 EDTA lavender top tube (capillary for automation).
|
| Minimum Volume/Size Required |
Fill to manufacturer’s stated fill volume. Minimum of 1mL in a 2mL vacuum tube or 375µL minimum in a 500µL capillary for automation tube.
|
| Storage/Preservation Prior to Shipping |
- Invert lavender top tubes at least 10 times immediately after collection to avoid clotting.
- Store at ambient or refrigerated temperature.
- Do not freeze.
- Avoid high temperature environments.
|
| Transport Medium |
N/A |
| Specimen Labeling |
- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number
- Identifiers on specimen must exactly match submission form.
|
| Shipping and Specimen Handling Requirements |
- Specimens may be shipped at ambient or refrigerated temperature
- Specimens must be received by the laboratory within 14 days from collection.
- Do not freeze specimens.
- Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
- Handle as infectious agent using universal precautions
- Ship triple-contained in accordance with federal shipping regulations for infectious agents.
|
| Method |
Flow cytometry |
| Turn-around Time |
4-5 working days after receipt of specimen |
| Interferences/Limitations |
Clotting, hemolysis, extremely elevated WBC’s, lipemia, abnormal proteins. |
| Common Causes for Rejection |
- Specimen is clotted.
- Two patient-specific identifiers not met.
- Specimen is collected in an expired blood collection tube.
- Specimen is not mailed or shipped in a timely or proper manner.
- Demographic information is missing or incorrect.
- Specimen identification is missing or incorrect.
- Specimen tube broken/leaked in transit.
- Submission form not included with specimen.
- No specimen included with submission form.
- Specimen identification illegible
- Incorrect blood collection tube used.
- Specimen volume is insufficient or excessive.
|
| Additional Information |
Reference range:
11.0 - 16.0 g/dL
Contact:
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