Serotyping Neisseria meningitidis

Laboratory Fee Schedule
Procedure #: MAA0013A
CPT: 87147

Synonym(s):	Meningitis serotyping
Requisition Form	G-2B
Test Description	Serotyping of invasive Neisseria meningitidis from sterile sites or purpuric lesions
Pre-Approval Needed	N/A
Supplemental Information Required	N/A
Supplemental Form(s)	N/A
Performed on Specimens from (sources)	Human
Sample/Specimen Type for Testing	Pure isolate
Minimum Volume/Size Required	At least one viable colony
Storage/Preservation Prior to Shipping	Slants: Inoculate cultures on chocolate agar slants and incubate overnight at @ 35-37oC in a 5% CO2 atmosphere. Store at ambient temperature. Frozen stocks: Collect the isolate’s overnight growth from the agar plate with a sterile swab and resuspend bacteria in an appropriate freezer medium (such as TSB with 15% glycerol or defibrinated sheep blood).
Transport Medium	Appropriate solid medium that supports growth
Specimen Labeling	Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements	Ship according to Dangerous Good Regulation, IATA, and/or CFR 49. Slants: Ship at ambient temperature. Frozen Stocks: Ship on dry ice.
Method	Conventional serotyping
Turn-around Time	3-10 days
Interferences/Limitations	Material must be viable. Improper storage or shipping temperatures limit organism recovery.
Common Causes for Rejection	Insufficient quantity Unacceptable specimen type or source Improper shipping conditions Expired media or collection container Discrepancies between specimen label and submission form Incomplete or missing submission form.
Additional Information	Isolates from sterile sites or purpuric lesions are required to be submitted per Texas Administrative code (RAC) chapter 97: §97.3(a)(4), §97.4(a)(6), and §97.5(a)(2)(C) Notifiable Conditions \| Texas DSHS