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Synonym(s):
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Meningitis
serotyping
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Requisition Form
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G-2B |
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Test Description
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Serotyping
of invasive Neisseria meningitidis from
sterile sites or purpuric lesions |
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Pre-Approval Needed
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N/A
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Supplemental Information Required
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N/A |
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Supplemental Form(s)
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N/A
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Performed on Specimens from (sources)
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Human
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Sample/Specimen Type for Testing
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Pure
isolate
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Minimum Volume/Size Required
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At least
one viable colony
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Storage/Preservation Prior to Shipping
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Slants: Inoculate cultures on chocolate agar slants and incubate overnight at @ 35-37oC in a 5% CO2 atmosphere. Store at ambient temperature. Frozen stocks: Collect the isolate’s overnight growth from the agar plate with a sterile swab and resuspend bacteria in an appropriate freezer medium (such as TSB with 15% glycerol or defibrinated sheep blood).
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Transport Medium
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Appropriate
solid medium that supports growth
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Specimen Labeling
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- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number
- Identifiers on specimen must exactly match submission form.
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Shipping and Specimen Handling Requirements
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- Ship according to Dangerous Good Regulation,
IATA, and/or CFR 49.
- Slants: Ship at ambient temperature.
- Frozen Stocks: Ship on dry ice.
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Method
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Conventional serotyping
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Turn-around Time
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3-10 days
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Interferences/Limitations
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-
Material must be viable.
- Improper storage or shipping temperatures limit
organism recovery.
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| Common Causes for Rejection
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-
Insufficient quantity
- Unacceptable
specimen type or source
- Improper shipping conditions
- Expired media or
collection container
- Discrepancies between specimen label and submission
form
- Incomplete or missing submission form.
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Additional Information
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Isolates
from sterile sites or purpuric lesions are required
to be submitted per Texas Administrative code (RAC) chapter 97: §97.3(a)(4), §97.4(a)(6), and §97.5(a)(2)(C) https://www.dshs.state.tx.us/idcu/investigation/conditions/
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