Synonym(s):
|
Meningitis
serotyping
|
Requisition Form
|
G-2B |
Test Description
|
Serotyping
of invasive Neisseria meningitidis from
sterile sites or purpuric lesions |
Pre-Approval Needed
|
N/A
|
Supplemental Information Required
|
N/A |
Supplemental Form(s)
|
N/A
|
Performed on Specimens from (sources)
|
Human
|
Sample/Specimen Type for Testing
|
Pure
isolate
|
Minimum Volume/Size Required
|
At least
one viable colony
|
Storage/Preservation Prior to Shipping
|
Slants: Inoculate cultures on chocolate agar slants and incubate overnight at @ 35-37oC in a 5% CO2 atmosphere. Store at ambient temperature. Frozen stocks: Collect the isolate’s overnight growth from the agar plate with a sterile swab and resuspend bacteria in an appropriate freezer medium (such as TSB with 15% glycerol or defibrinated sheep blood).
|
Transport Medium
|
Appropriate
solid medium that supports growth
|
Specimen Labeling
|
- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number
- Identifiers on specimen must exactly match submission form.
|
Shipping and Specimen Handling Requirements
|
- Ship according to Dangerous Good Regulation,
IATA, and/or CFR 49.
- Slants: Ship at ambient temperature.
- Frozen Stocks: Ship on dry ice.
|
Method
|
Conventional serotyping
|
Turn-around Time
|
3-10 days
|
Interferences/Limitations
|
-
Material must be viable.
- Improper storage or shipping temperatures limit
organism recovery.
|
Common Causes for Rejection
|
-
Insufficient quantity
- Unacceptable
specimen type or source
- Improper shipping conditions
- Expired media or
collection container
- Discrepancies between specimen label and submission
form
- Incomplete or missing submission form.
|
Additional Information
|
Isolates
from sterile sites or purpuric lesions are required
to be submitted per Texas Administrative code (RAC) chapter 97: §97.3(a)(4), §97.4(a)(6), and §97.5(a)(2)(C) https://www.dshs.state.tx.us/idcu/investigation/conditions/
|