| Synonym(s): | Nocardia, Actinomadura, filamentous bacteria |
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| Requisition Form | G-MYCO |
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| Test Description | Identification of Nocardia species, Actinomadura species, etc. |
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| Pre-Approval Needed | No |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Pure isolate from human sources. |
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| Sample/Specimen Type for Testing | Pure isolate in liquid or on solid culture medium. |
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| Minimum Volume/Size Required | - Liquid culture: 2.0 mL
- Solid culture: Visible growth
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| Storage/Preservation Prior to Shipping | Ambient |
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| Transport Medium | N/A |
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| Specimen Labeling | - At least two patient identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements | - Ship at ambient temperatures.
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply to unidentified isolates.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Additional DSHS Laboratory specimen shipping guidelines online here.
- Ship specimens overnight Monday–Thursday to avoid weekend deliveries.
- Do not ship specimens for holiday or weekend delivery.
- Ensure media used to isolate specimens is not expired.
- Additional DSHS Laboratory submission guidelines available online here.
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| Method | Biochemical and physiological testing |
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| Turn-around Time | 21–28 days. |
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| Interferences/Limitations | Some genera and species can only be differentiated with molecular analysis that is not available at TDSHS. |
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| Common Causes for Rejection | - Cultures infested with mites.
- Cultures broken in transit.
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| Additional Information | - Isolates submitted for this test will be identified by this laboratory.
- Actinomycete drug susceptibility testing is not available at DSHS Laboratory.
- Upon request, isolates may be forwarded by the DSHS Laboratory to a reference laboratory for susceptibility testing at the submitter’s expense.
- Testing at other reference laboratories is subject to fees charged by those laboratories.
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