Accuprobe

AFB Isolate Identification by Probe

Laboratory Fee Schedule

Procedure: MAC0053A

CPT: 87149

**AFB Isolate Identification by Probe**
Synonym(s):	Accuprobe, DNA probe
Requisition Form	G-MYCO
Test Description	Identification Mycobacterium tuberculosis complex, Mycobacterium gordonae, Mycobacterium avium complex, Mycobacterium kansasii by DNA probe
Pre-Approval Needed	No
Supplemental Information Required	N/A
Supplemental Form(s)	N/A
Performed on Specimens from (sources)	Pure isolate from human sources
Sample/Specimen Type for Testing	Pure isolate in liquid or on solid culture medium
Minimum Volume/Size Required	• Liquid culture 2.0 mL • Solid culture - Visible growth
Storage/Preservation Prior to Shipping	Ambient
Transport Medium	N/A
Specimen Labeling	• Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number) • Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements	• Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49 • Category B shipping applies for unidentified isolates
Method	Accuprobe culture confirmation test; hybridization of ribosomal RNA to probe detected by chemiluminesence
Turn-around Time	10 to 14 days for final report when included as part of a referred isolate identification
Interferences/Limitations	• Positive DNA Probe test does not mean the isolate is still viable.
Common Causes for Rejection	• Cultures broken in transit • Culture completely overgrown by mold
Additional Information	• Drug susceptibility tesing on M. avium complex and M. gordonae not available at TDSHS laboratory. Isolates may be forwarded by TDSHS laboratory to reference laboratory for susceptibility testing. A handling fee may be charged. Testing at other reference laboratories is subject to fees charged by those laboratories.