Acid Fast Bacilli Biochemicals, Complex

Laboratory Fee Schedule

Procedure: MZZ0455A  

CPT: 87118

Synonym(s): Bios, Complex
Requisition Form G-MYCO
Test Description Identification of mycobacterial isolates referred to DSHS Laboratory by biochemical tests.
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A  
Performed on Specimens from (sources) Pure isolate from human sources.
Sample/Specimen Type for Testing Mycobacterium species pure isolate in liquid or on solid culture medium
Minimum Volume/Size Required

Liquid culture - 2.0 mL

Solid culture - Single mature colony

Storage/Preservation Prior to Shipping Ambient
Transport Medium N/A
Specimen Labeling

At least 2 patient specific identifiers:  First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. medical records number).

Specimen identification must match specimen submission form.

Shipping and Specimen Handling Requirements

Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49

Category B shipping applies for unidentified isolates.


Biochemical testing to detect the presence of absence of an enzyme.

Physiological testing

Turn-around Time 14 days.
  • Identification by biochemical tests is performed in conjunction with the morphological characteristics of the isolate and the HPLC pattern derived from cell wall analysis.
  • Biochemical testing can be affected by the age of the culture.
  • Identification by biochemical testing is limited and may not differentiate species without testing such as sequencing or mass spectrometry.
Common Causes for Rejection
  • Cultures overgrown by mold.
  • Cultures broken in transit.
Additional Information

Complex biochemical testing includes the most common biochemical panel and the less frequently used biochemical/physiological studies for the identification of Mycobacterium species.