Laboratory Fee Schedule
Procedure #: MCK0290Z
CPT: 87076
| Synonym(s): | Anaerobe ID, Anaerobic bacteria, culture |
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| Requisition Form | G-2B |
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| Test Description | Identification ofanaerobic bacteria from pure anaerobic isolate |
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| Pre-Approval Needed | N/A |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Human |
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| Sample/Specimen Type for Testing | Pure anaerobic isolate under anaerobic conditions |
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| Minimum Volume/Size Required | At least one viable colony |
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| Storage/Preservation Prior to Shipping | Store anaerobically at room temperature (15°C–25°C) or at refrigeration temperatures (2°C–8°C). |
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| Transport Medium | Any medium that supports growth in anaerobic conditions (e.g. Chopped Meat Glucose broth, thioglycolate broth, media in anaerobe pouch, or frozen in TSB + glycerol. |
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| Specimen Labeling | - At least two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three unique patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements | - Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Ship overnight at ambient temperatures if isolate was stored at room temperature.
- Ship overnight on cold packs to Laboratory if isolate was stored cold.
- Ship overnight frozen on dry ice if isolate was stored frozen.
- Ship specimens overnight Monday–Thursday to avoid weekend deliveries.
- Do not ship specimens for holiday or weekend delivery.
- Ensure media used to ship isolates are not expired.
- Additional DSHS Laboratory specimen shipping guidelines online here.
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| Method | MALDI-TOF (mass spectrometry), Bacterial culture |
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| Turn-around Time | 2-14 days |
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| Interferences/Limitations | - Isolates must be viable.
- Improper storage or shipping temperatures limit organism recovery.
- In the event of an outbreak, the procedure may be modified to screen for an additional set of organisms.
- Where a specific agent of an outbreak has been previously identified and there is a backlog of specimens, the procedure may be modified to screen only for specified organism.
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| Common Causes for Rejection | - insufficient specimen (non-viable/no growth)
- improperly stored
- improper shipping conditions
- expired media or collection container
- discrepancies between specimen label and submission form
- incomplete or missing submission form
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| Additional Information | N/A |
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