Brucella Total Antibodies Agglutination Test


Laboratory Fee Schedule

Procedure: MZZ0135A

CPT: 86622

Brucella Total Antibodies Agglutination Test


DS Microtiter Procedure for Brucella Agglutination Test

Requisition Form


Test Description

The detection and quantitation of B. abortus IgG and IgM antibodies by the agglutination of safranin-O stained whole cells

Pre-Approval Needed


Supplemental Information Required


Supplemental Form(s)


Performed on Specimens from (sources)


Sample/Specimen Type for Testing

  • Serum

Minimum Volume/Size Required

1.0 mL

Storage/Preservation Prior to Shipping

  • Blood should be allowed to clot at room temperature then centrifuged.

  • Serum can be used if particulates are removed via filtration or by using lipid clearing reagents and centrifugation.

  • Serum can be stored at  2°C  to 8°C  if tested within two days or should be Frozen at ≤ -15°C or colder if not tested within 2 days.

Transport Medium


Specimen Labeling

  • Two patient-specific identifiers required (e.g. patient full name, date of birth, Medical Record number)

  • Identifiers on specimen must exactly match submission form.

Shipping and Specimen Handling Requirements

  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.

  • Handle as infectious agent using universal precautions. 

  • Triple contain in accordance with federal shipping regulations for infectious agents.
    Additional forms & resources


Antibody agglutination test

Turn-around Time

3 working days


Hemolytic, hyperlipemic and bacterial contamination can produce erroneous results in serologic tests.

Common Causes for Rejection

  • Insufficient quantity

  • Unacceptable specimen type or source

  • Improper shipping conditions

  • Expired media or collection container

  • Discrepancies between specimen label and submission form

  • Incomplete or missing submission form.

Additional Information

This test can provide presumptive evidence of Brucellosis.  Results should be considered in combination with other clinical, laboratory and epidemiologic findings.