Brucella Total Antibodies Agglutination Test

Laboratory Fee Schedule
Procedure: MZZ0135A
CPT: 86622


DS Microtiter Procedure for Brucella Agglutination Test

Requisition Form


Test Description

The detection and quantitation of B. abortus IgG and IgM antibodies by the agglutination of safranin-O stained whole cells

Pre-Approval Needed


Supplemental Information Required


Supplemental Form(s)


Performed on Specimens from (sources)


Sample/Specimen Type for Testing

  • Serum

Minimum Volume/Size Required

1.0 mL

Storage/Preservation Prior to Shipping

  • Blood should be allowed to clot at room temperature then centrifuged.

  • Serum can be used if particulates are removed via filtration or by using lipid clearing reagents and centrifugation.

  • Serum can be stored at  2°C  to 8°C  if tested within two days or should be Frozen at ≤ -15°C or colder if not tested within 2 days.

Transport Medium


Specimen Labeling

  • Two patient-specific identifiers required (e.g. patient full name, date of birth, Medical Record number)

  • Identifiers on specimen must exactly match submission form.

Shipping and Specimen Handling Requirements

  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.

  • Handle as infectious agent using universal precautions. 

  • Triple contain in accordance with federal shipping regulations for infectious agents.
    Additional forms & resources


Antibody agglutination test

Turn-around Time

3 working days


Hemolytic, hyperlipemic and bacterial contamination can produce erroneous results in serologic tests.

Common Causes for Rejection

  • Insufficient quantity

  • Unacceptable specimen type or source

  • Improper shipping conditions

  • Expired media or collection container

  • Discrepancies between specimen label and submission form

  • Incomplete or missing submission form.

Additional Information

This test can provide presumptive evidence of Brucellosis.  Results should be considered in combination with other clinical, laboratory and epidemiologic findings.