Burkholderia PCR

Synonym(s):

Melioidosis or Burkholdeia pseudomallei, glanders or Burkholderia mallei PCR

Requisition Form

G-27A

Test Description

Detection of Burkholderia mallei/pseudomallei DNA by PCR

Pre-Approval Needed

Must notify BioThreat Team prior to sample submission: (512) 689-5537

Supplemental Information Required

N/A

Supplemental Form(s)

N/A

Performed on Specimens from (sources)

• Non-Human (Bacterial)

• Human

• Environmental 

Sample/Specimen Type for Testing

• Culture isolates

• Whole blood

• Serum

• Environmental specimens

Minimum Volume/Size Required

Culture isolates

• 1 plate or slant

Clinical samples

• 1 mL

Storage/Preservation Prior to Shipping

 Culture isolates

• Ambient

Clinical or environmental samples

• 2-8 °C (cold)

Transport Medium

N/A

Specimen Labeling

Human

• Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)

• Identifiers on specimen must exactly match submission form.

Non-Human or Environmental

• Sample identification must match submission form.

Shipping and Specimen Handling Requirements

• Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.

• Handled as infectious agent using universal precautions. 

• Triple contained in accordance with federal shipping regulations for infectious agents. 

• See also Emergency Preparedness resources online..

Notify BioThreat Team prior to sample submission: (512) 689-5537

Method

PCR

Turn-around Time

Preliminary result 1 day after receipt, final result 1- 21 days

Interferences/Limitations

Improper storage or shipping temperatures may limit organism recovery.

Common Causes for Rejection

• Insufficient quantity

• Unacceptable specimen type or source

• Improper shipping conditions

• Expired media or collection container

• Discrepancies between specimen label and submission form

• Incomplete or missing submission form

Additional Information

May be paired with Definitive ID: Brucella species