Direct Detection by High Performance Liquid Chromatography (HPLC)

Laboratory Fee Schedule
Procedure: MAF0064A
CPT: 87143

Direct Detection by High Performance Liquid Chromatography (HPLC)


Direct HPLC

Requisition Form


Test Description

Mycolic acid analysis identification of AFB in smear-positive processed specimens

Pre-Approval Needed

Yes; call 512-776-7342 for pre-approval

Supplemental Information Required


Supplemental Form(s)


Performed on Specimens from (sources)

Clinical specimens from humans

Sample/Specimen Type for Testing

Specimens must be concentrated and smear positive

Minimum Volume/Size Required

Processed sediment volume >/= 1 mL

Storage/Preservation Prior to Shipping


Transport Medium


Specimen Labeling

  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)

  • Identifiers on specimen must exactly match submission form.

Shipping and Specimen Handling Requirements

  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49

  • Category B shipping applies


High performance liquid chromatography

Turn-around Time

1 day


  • Direct HPLC may only detect the predominant organism in a mixed population.

  • Direct HPLC is not highly sensitive and will not be conclusive on specimens that have negative or weakly AFB positive smears.

  • Direct HPLC cannot determine the viability of organisms detected

  • Direct HPLC results that are negative for M. tuberculosis complex do not conclusively rule out the presence of viable M. tuberculosis complex in the specimen.

Common Causes for Rejection

Volume insufficient for testing

Additional Information

Processed specimen sediments may be accepted for testing following pre-approval but AFB smear and culture must be performed concurrently in the submitting laboratory.