Shiga-like toxin, ST-1 ST-2, STEC, EHEC, E. coli toxin

G-2B

Escherichia coli shiga toxin assay

N/A

N/A

N/A

Human

Pure culture E. coli; Clinical stool

Pure culture

• At least one viable colony

Clinical sample

• 500 µl

Pure Culture: 

• Refrigerated

Broth/liquid transport

• Refrigerated: Received < 7 days
• Frozen @-70C: Received > 7 days.

• Media supporting growth of isolate.
• Clinical sample in GN broth or transport media

• Two patient-specific identifiers required (e.g., patient full name, date of birth, medical record number)
• Identifiers on specimen must exactly match submission form.

• Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
• Ship specimens overnight Monday-Thursday, to avoid weekend deliveries.

Enzyme Immunoassay

3-21 days

• Material must be viable.  
• Improper storage or shipping temperatures limit organism recovery. 

• Insufficient quantity
• Unacceptable specimen type or source
• Improper shipping conditions
• Expired media or collection container
• Discrepancies between specimen label and submission form
• Incomplete or missing submission form.

N/A