Haemophilus spp. identification
Laboratory Fee Schedule
Procedure #: MAA0034A
CPT: 87077,
| Synonym(s): | Haemophilus influenza, H. flu., Hi |
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| Requisition Form | G-2B |
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| Test Description | Identification of Haemophilus sp. |
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| Pre-Approval Needed | N/A |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Human |
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| Sample/Specimen Type for Testing | Pure isolate |
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| Minimum Volume/Size Required | At least one viable colony |
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| Storage/Preservation Prior to Shipping | - Incubate overnight at 35°C–37°C with ~5% CO2.
- Store slants at an room temperature (15°C–25°C).
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| Transport Medium | Appropriate solid medium that supports growth |
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| Specimen Labeling | - At least two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three unique patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements | - Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Additional DSHS Laboratory specimen shipping guidelines online here.
- Ship specimens overnight at room temperature (15°C–25°C).
- Ship specimens overnight Monday–Thursday to avoid weekend deliveries.
- Do not ship specimens for holiday or weekend delivery.
- Ensure media used for isolate are not expired.
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| Method | Bacterial culture, MALDI-TOF (mass spectrometry), conventional serotyping (Haemophilus influenzae from sterile sites) |
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| Turn-around Time | 2–21 days |
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| Interferences/Limitations | - Isolates must be viable.
- Improper storage or shipping temperatures limit organism recovery.
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| Common Causes for Rejection | - insufficient quantity
- unacceptable specimen type or source
- improper shipping conditions
- expired media or collection container
- discrepancies between specimen label and submission form
- incomplete or missing submission form
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| Additional Information | Isolates from sterile sites identified as Haemophilus influenza will be serotyped. |
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