Laboratory Fee Schedule

Procedure: BZZ0024A

CPT: 85018


Synonym(s): Total Hemoglobin, Hgb
Requisition Form G-1B, G-THSTEPS
Test Description The measurement of hemoglobin is used to detect anemia in whole blood specimens.
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Human
Sample/Specimen Type for Testing
  • Preferred:  Whole blood in (BD) K2 EDTA lavender top vacuum tube (venous).
  • Acceptable:  Whole blood in (BD MAP) K2 EDTA lavender top tube (capillary for automation).
Minimum Volume/Size Required

Fill to manufacturer’s stated fill volume. Minimum of 1mL in a 2mL vacuum tube or 375µL minimum in a 500µL capillary for automation tube.

Storage/Preservation Prior to Shipping
  • Invert lavender top tubes at least 10 times immediately after collection to avoid clotting.
  • Store at ambient or refrigerated temperature.
  • Do not freeze.
  • Avoid high temperature environments.
Transport Medium N/A
Specimen Labeling
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number
  • Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements
  • Specimens may be shipped at ambient or refrigerated temperature
  • Specimens must be received by the laboratory within 14 days from collection.  
  • Do not freeze specimens.
  • Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
  • Handle as infectious agent using universal precautions
  • Ship triple-contained in accordance with federal shipping regulations for infectious agents. 
Method Flow cytometry
Turn-around Time 4-5 working days after receipt of specimen
Interferences/Limitations Clotting, hemolysis, extremely elevated WBC’s, lipemia, abnormal proteins.
Common Causes for Rejection
  • Specimen is clotted.
  • Two patient-specific identifiers not met.
  • Specimen is collected in an expired blood collection tube. 
  • Specimen is not mailed or shipped in a timely or proper manner. 
  • Demographic information is missing or incorrect. 
  • Specimen identification is missing or incorrect.
  • Specimen tube broken/leaked in transit. 
  • Submission form not included with specimen.
  • No specimen included with submission form. 
  • Specimen identification illegible
  • Incorrect blood collection tube used. 
  • Specimen volume is insufficient or excessive.
Additional Information

Reference range:

11.0 - 16.0 g/dL