Laboratory Fee Schedule
Procedure: MBK0213A
CPT: 87340
| Synonym(s): | HBsAg, HBV, HBAG |
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| Requisition Form | G-2A |
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| Test Description | A qualitative enzyme immunoassay for the detection of Hepatitis B Surface Antigen (HBsAg) in human serum. |
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| Pre-Approval Needed | N/A |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Human |
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| Sample/Specimen Type for Testing | Serum |
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| Minimum Volume/Size Required | 500 µL |
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| Storage/Preservation Prior to Shipping | - Store serum at 2°C to 8°C for up to 48 hours.
- For storage longer than 48 hours, store at -20°C or below until tested.
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| Transport Medium | N/A |
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| Specimen Labeling | - Two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, medical record number)
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form (e.g., abbreviated name on label and full name on form is not acceptable).
- Additional DSHS Laboratory specimen labeling guidelines available online here.
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| Shipping and Specimen Handling Requirements | - Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Handle as infectious agent using universal precautions.
- Triple-contain in accordance with federal shipping regulations for infectious agents.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- If specimens will arrive at the Lab within 5 days of collection, refrigerate at 2°C–8°C and ship overnight on cold packs.
- If specimens will arrive at the Lab more than 5 days after date of collection, freeze at -20°C or below, and ship overnight on dry ice.
- Additional DSHS Laboratory specimen shipping guidelines online here.
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| Method | Enzyme-Linked Immunosorbent Assay (ELISA) |
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| Turn-around Time | 4 working days |
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| Interferences/Limitations | - A person who has been vaccinated for hepatitis B may have transient reactivity for up to 18 days.
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| Common Causes for Rejection | - Insufficient specimen quantity.
- Unacceptable specimen type or source.
- Improper shipping conditions.
- Expired media or collection container.
- Expired reagents/media/collection container.
- Discrepancies between G-2A submission form and specimen label.
- Missing/incomplete/illegible submission form or label.
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| Additional Information | - A designation of reactive for HBsAg must not be based on a single reactive test result. Reactive specimens will automatically reflex to confirmatory testing.
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