Laboratory Fee Schedule
Procedure #: MBP0232A
CPT: 87536

 Synonym(s):   HIV-1 RNA Quantification, HIV-1 NAAT, HIV-1 RNA 
 Requisition Form  G-2A
 Test Description  Nucleic Acid Amplification Test (NAAT) to detect and quantify Human Immunodeficiency Virus type 1 (HIV-1) RNA in serum and plasma 
 Pre-Approval Needed  N/A
 Supplemental Information Required  N/A
 Supplemental Form(s)  N/A
 Performed on Specimens from (sources)  Human
 Sample/Specimen Type for Testing
  • Serum collected in Serum Separator tubes (SST) or red top tubes
  • Plasma collected in purple top tubes with EDTA
 Minimum Volume/Size Required 1200µL of plasma or serum 
 Storage/Preservation Prior to Shipping
  • Serum
    • Store at 2°C to 8°C for up to 5 days
    • Store at -15°C to -35°C for up to 90 days
  • Plasma
    • Store at 2°C to 8°C for up to 5 days.
    • Store at -20°C to -70°C for up to 90 days 
 Transport Medium  N/A
 Specimen Labeling
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match submission form.
 Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
  • Handle as infectious agent using universal precautions.
  • Triple contained in accordance with federal shipping regulations for infectious agents.
    Additional forms & resources
 Method  RNA Quantification by Real-Time Transcription Mediated Amplification
 Turn-around Time  3 working days
  • Reliable results are dependent on adequate specimen collection, transport, storage, and processing.  
  • This assay has been validated for use as a quantitative assay with plasma only.
  • This assay has been validated for use as a qualitative assay with plasma and serum
  • Though rare, mutations within the highly conserved regions of the viral genome covered by the primers and/or probes in this assay may result in underquantification of or failure to detect the virus.
 Common Causes for Rejection
  • Insufficient quantity
  • Unacceptable specimen type or source
  • Improper shipping conditions
  • Expired media or collection container
  • Discrepancies between specimen label and submission form
  • Incomplete or missing submission form
 Additional Information  N/A