HIV-1 Western Blot Dried Blood Spot (DBS)
|HIV-1 Western Blot Dried Blood Spot (DBS)
|Proteins from HIV-1 are separated using gel electrophoresis then trans-blotted to nitrocellulose sheets. Conjugate is added and if specific HIV-1 antibodies are present, they will bind to the HIV-1 proteins.
|Supplemental Information Required
|Performed on Specimens from (sources)
|Sample/Specimen Type for Testing
|Dried blood spot
|Minimum Volume/Size Required
|¼ inch of dried blood spot
|Storage/Preservation Prior to Shipping
|Stored at 2°C to 8°C or frozen at -20°C for at least two months with low humidity (<60%)
|At least 2 patient specific identifiers: First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. Medical Record number); must match information on submission form (G-2A).
|Shipping and Specimen Handling Requirements
|Material must be viable. Improper storage or shipping temperatures limit organism recovery.
|Causes for Rejection
|Insufficient quantity, unacceptable specimen type or source, improper shipping conditions, expired media or collection container, discrepancies between specimen label and submission form, and/or incomplete or missing submission form.