HIVdbs
HIV-1 Western Blot Dried Blood Spot (DBS)
Procedure: MBQ0232A
| Synonym(s): | HIV-1 Western Blot Dried Blood Spot (DBS) |
|---|---|
| Requisition Form | G-2A |
| Test Description | Proteins from HIV-1 are separated using gel electrophoresis then trans-blotted to nitrocellulose sheets. Conjugate is added and if specific HIV-1 antibodies are present, they will bind to the HIV-1 proteins. |
| Pre-Approval Needed | N/A |
| Supplemental Information Required | N/A |
| Supplemental Form(s) | N/A |
| Performed on Specimens from (sources) | Human |
| Sample/Specimen Type for Testing | Dried blood spot |
| Minimum Volume/Size Required | ¼ inch of dried blood spot |
| Storage/Preservation Prior to Shipping | Stored at 2°C to 8°C or frozen at -20°C for at least two months with low humidity (<60%) |
| Transport Medium | N/A |
| Specimen Labeling | At least 2 patient specific identifiers: First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. Medical Record number); must match information on submission form (G-2A). |
| Shipping and Specimen Handling Requirements |
|
| Method | Protein detection |
| Turn-around Time | N/A |
| Interferences/Limitations | Material must be viable. Improper storage or shipping temperatures limit organism recovery. |
| Causes for Rejection | Insufficient quantity, unacceptable specimen type or source, improper shipping conditions, expired media or collection container, discrepancies between specimen label and submission form, and/or incomplete or missing submission form. |
| Additional Information | N/A |