HPLC Identification of clinical isolates

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HPLC Identification of clinical isolates

Acid Fast Bacilli Isolate Identification by High 

Performance Liquid Chromatography (HPLC)   

Laboratory Fee Schedule

Procedure: MAC0052A

CPT: 87143

Synonym(s): HPLC Identification of clinical isolates
Requisition Form G-MYCO
Test Description Mycolic acid analysis identification of mycobacterial isolates cultured from clinical specimens in DSHS Laboratory
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A  
Performed on Specimens from (sources) Pure isolate from human sources.
Sample/Specimen Type for Testing Mycobacterium species recovered by DSHS laboratory in liquid or on solid culture medium.
Minimum Volume/Size Required
  • Liquid culture- 2.0 mL
  • Solid culture- Single mature colony
Storage/Preservation Prior to Shipping N/A
Transport Medium N/A
Specimen Labeling
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, medical record number)
  • Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements

N/A
Method High performance liquid chromatography
Turn-around Time 2 days.
Interferences/Limitations
  • HPLC may only detect the predominant organism in a mixed culture.
  • Liquid cultures must be instrument positive in order to analyze by HPLC.
  • Additional testing methods may need to be used to provide definitive identification.
Common Causes for Rejection N/A
Additional Information

This test is used in-house to identify Mycobacterium species cultured from submitted clinical specimens.