Measles Virus Detection, Real-Time RT-PCR

Laboratory Fee Schedule
Procedure #:  MZZ0750A
CPT:  87798

Synonym(s):PCR, Rubeola
Requisition FormG-2V
Test DescriptionTesting of swab specimens from suspected measles cases to detect
the presence of measles virus RNA in symptomatic patients.
Pre-Approval NeededYes. Contact your local or state epidemiologist if measles is
suspected. Contact information can be found at the following link:
http://www.dshs.texas.gov/idcu/investigation/conditions/contacts/
Supplemental Information RequiredDate of onset
Supplemental Form(s)N/A
Performed on Specimens from (sources)Human
Sample/Specimen Type for Testing
  • Preferred: Throat swabs
  • Acceptable: Nasopharyngeal swabs 
  • Specimens must be collected using only swabs with a synthetic tip, such as nylon, Dacron, rayon and an aluminum or plastic shaft.
  • Do not use swabs with cotton tips or wooden shafts.
     
Minimum Volume/Size Required1 mL –2 mL
Storage/Preservation Prior to Shipping
  • Store and ship cold at 2°C–8°C if specimen will arrive at the Laboratory within 48 hours of collection. 
  • Store and ship frozen at -70°C or colder if specimen will arrive at the Laboratory more than 48 hours after collection.
     
Transport Medium
  • Universal transport media (UTM)
  • Viral transport media (VTM)
Specimen Labeling
  • At least two patient-specific identifiers required on specimen (e.g. patient full name, date of birth, medical record number). 
  • Three unique patient identifiers are preferred.  
  • Patient identifiers on specimen label must exactly match identifiers on G-2V submission form. (e.g. abbreviated name on label and full name on form is not acceptable.) 
  • Additional DSHS Laboratory specimen labeling guidelines available online here. 
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49. 
  • Category B Biological Substance, UN3373 shipping requirements apply. 
  • Submitters are responsible for packaging and shipping specimens according to regulatory requirements.  
  • Store and ship cold at 2°C–8°C if specimen will arrive at the Laboratory within 48 hours of collection. 
  • Store and ship frozen at -70°C or colder if specimen will arrive at the Laboratory more than 48 hours after collection.
  • Additional DSHS Laboratory specimen shipping guidelines online here
MethodReal-time RT-PCR
Turn-around Time2 business days
Interferences/Limitations
  • Calcium alginate swabs are unacceptable.
  • Cotton swabs with wooden shafts are not recommended as they interfere with testing.
  • False negative may result if specimen is improperly collected, transported, or handled.
  • Detection of viral RNA does not imply that the corresponding virus is infectious or is the causative agent for clinical symptoms.
  • A negative result does not preclude measles infection.
Common Causes for Rejection
  • Patient identifiers on the specimen and on the G-2V submission
    form do not match.
  • Improper shipping conditions.
  • Expired media or collection container.
  • Discrepancies between specimen label and submission form.
  • Incomplete, illegible, or missing submission form.
Additional InformationN/A