Microbiology Laboratory Tests: Anaerobic Bacterial Culture to Aspergillosis
Microbiology
Anaerobic Bacterial Culture, Special (Isolation)
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| Test Includes: Rapid ANA II, API AnaID, GLC, conventional biochemicals | |
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Reporting |
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| Results Available: 7-14 days | Contact #s: (512) 458-7582 |
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Reference |
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| Method: Rapid ANA II, API AnaID, GLC, conventional biochemicals | |
| Turnaround Time: 7-14 days | Reference Range: No anaerobes isolated |
| Limitations: Transport method must be suitable for anaerobic conditions or organisms may not be viable for testing. Culture results may require additional clinical information about how specimen was obtained. Since many of these organisms are members of endogenous flora of the skin, oral cavity, genital tract, and gut, contamination by the normal flora during specimen acquisition is often a problem. | Interpretation: Interpretation of culture results may require additional clinical information about how the specimen was obtained. |
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Specimen Requirements |
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| Specimen Collection: Needle aspirates, abscess drainage. Swab collection is discouraged. | Sample Type: Deep wounds; sterile fluids; abscess material. |
| Volume/Amount Required: aspirates or drainage >1mL. Tissues: small piece | Preferred Specimen: Deep wounds, sterile fluids, abscess material, tissue in anaerobe transport (stools for botulism only) |
| Collection/Preservation: Must be collected and shipped under anaerobic conditions. | Storage Instructions: Store under anaerobic conditions. |
| Causes for Rejection: Shipped under aerobic conditions; no identifying markers on sample and/or paperwork, | Sample Container: Sterile specimen collector designed to maintain anaerobic atmosphere |
| Sample Test Kit: Rapid ANA, API ANAID | Availability: Tested Monday – Friday |
| Diagnostic Information: Please indicate suspected organism(s) when submitting specimen. Culture includes the identification of the predominant organism or pathogen(s). For Clostridium botulinum see Botulism Culture –(Isolation). | |
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Specimen Submission |
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| Required Request Form: G-2B | Specimen Handling: Handled as body fluids using universal precautions. |
| Transport Temperature: 2-8° C | Shipping Requirements: Triple contained in accordance with federal shipping regulations for diagnostic specimens. |
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Billing |
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| CPT Code: 87073, 87075 |
Fees: |
Microbiology
Anaerobic Bacterial Toxin Detection (Isolation)
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| Test Includes: Small animal inoculation, conventional biochemicals, Rapid ANA, API anaID | |
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Reporting |
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| Results Available: 2-10 days | Contact #s: (512) 458-7185 |
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Reference |
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| Method: Small animal inoculation, conventional biochemicals, Rapid ANA, API anaID | |
| Turnaround Time: 2-10 days | Reference Range: No toxin detected |
| Limitations: Ability to determine presence of toxin is dependent on ability to demonstrate protection with antitoxin. | Interpretation: No toxin detected indicates that there was no detectable toxin present in the specimen for Clostridium botulinum. This does not preclude other bacterial toxins. |
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Specimen Requirements |
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| Specimen Collection: venipuncture, bowel movement, water enema | Sample Type: Stool, serum |
| Volume/Amount Required: 15-20 mL serum 25-50 g stool; infant botulism: 2 mL serum, as much stool as possible. |
Preferred Specimen: Stool, Serum |
| Collection/Preservation: Specimens | Storage Instructions: 2-8° C |
| Causes for Rejection: Clinical history not consistent with botulism or no clinical history provided. Insufficient sample. | Sample Container: Tightly sealed, sterile leak proof container; sterile screw cap tube for serum |
| Sample Test Kit: Rapid ANA; API anaID | Availability: Routinely Monday-Friday, With telephone notification Saturday-Sunday |
| Diagnostic Information:Clostridium botulinum, see Botulism Culture –(Isolation) | |
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Specimen Submission |
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| Required Request Form: G-2B | Specimen Handling: Handled as infectious and toxic agent using universal precautions. (C botulinum toxin is one of the most powerful natural toxins known) |
| Transport Temperature: 2-8°C | Shipping Requirements: Overnight shipment, Triple contained in accordance with federal shipping regulations for diagnostic specimens. |
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Billing |
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| CPT Code: 87001 | Fees: |
Microbiology
Anaerobic Bacterial Toxin Detection (Identification)
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| Test Includes: Small animal inoculation, conventional biochemicals, Rapid ANA, API anaID | |
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Reporting |
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| Results Available: 2-10 days | Contact #s: (512) 458-7185 |
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Reference |
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| Method: Small animal inoculation, conventional biochemicals, Rapid ANA, API anaID | |
| Turnaround Time: 2-10 days | Reference Range: No toxin detected |
| Limitations: Culture must be viable | Interpretation: No toxin detected indicates that there was no detectable toxin present in the specimen. |
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Specimen Requirements |
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| Specimen Collection: n/a | Sample Type: Pure culture |
| Volume/Amount Required: one isolate per patient | Preferred Specimen: Pure cultures, 24 hour growth |
| Collection/Preservation: Isolated should be maintained for viability until shipment has been verified. | Storage Instructions: ambient temperature |
| Causes for Rejection: Specimen broken, transported aerobically. | Sample Container: Anaerobic transport tube or plate system. |
| Sample Test Kit: Rapid ANA, API anaID | Availability: Test performed Monday-Friday |
| Diagnostic Information: Clostridium botulinum see Botulism Culture (Toxin Detection), see Tetanus (Culture – Identification) | |
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Specimen Submission |
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| Required Request Form: G-2B | Specimen Handling: Infectious agent |
| Transport Temperature: Ambient temperature | Shipping Requirements: Triple contained in accordance with federal shipping regulations for infectious agents. |
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Billing |
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| CPT Code: 87001 | Fees: |
Microbiology
Anthrax Culture (Isolation)
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| Test Includes: Conventional biochemical methods, PCR, DFA | |
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Reporting |
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| Results Available: 3-5 days | Contact #s: (512) 776-3781 |
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Reference |
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| Method: Conventional biochemical methods, PCR, DFA | |
| Turnaround Time: Preliminary 1 day after receipt; Final 3-5 days after receipt | Reference Range: None isolated |
| Limitations: Quality of specimen collection will affect the ability to isolate pathogenic organisms from clinical specimens. | Interpretation: |
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Specimen Requirements |
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| Specimen Collection: Intestinal specimens: Should be obtained early in course of illness. Freshly passed stools are better than rectal swabs. | Sample Type: Exudate; Blood; Sputum; CSF; Tissue |
| Volume/Amount Required: | Preferred Specimen: Exudate; Blood; Sputum |
| Collection/Preservation: | Storage Instructions: Do not freeze or refrigerate whole blood. Keep stools, tissue, body fluids, wound exudates, and sputum at 2-8°C. |
| Causes for Rejection: Insufficient amount of sample to test due to leakage or other circumstances; no identifying marks on sample and/or paperwork; name discrepancies. | Sample Container: Stool- sterile feces container Rectal swab: Aimes, Stuart’s or Cary Blair transport tube Blood: Tiger top vacutainer Body fluids: Sterile collection tube |
| Sample Test Kit: | Availability: Tested Monday-Friday. With notification Saturday and Sunday. |
| Diagnostic Information: Prior notification is requested. (512) 776-3781. Bioterrorism Investigation Section. CDC serology available only with prior approval and with submission of patient history. Reports of confirmed Bacillus anthracis will be called to the submitter immediately upon completion of testing. Please insure that an accurate telephone number and contact name is included on the submission form. For suspected Bioterrorism agents, see Bioterrorism agents (Clinical -Isolation). | |
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Specimen Submission |
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| Required Request Form: G-27A | Specimen Handling: Universal precautions |
| Transport Temperature: 2-8°C | Shipping Requirements: Triple contained in accordance with federal shipping regulations for diagnostic specimens. |
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Billing |
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| CPT Code: 87076 | Fees: |
Microbiology
Anthrax Culture (Identification)
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| Test Includes: Conventional biochemical methods, PCR, DFA | |
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Reporting |
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| Results Available: 3-5 days | Contact #s: (512) 776-3781 |
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Reference |
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| Method: Conventional biochemical methods, PCR, DFA | |
| Turnaround Time: Preliminary 1 day after receipt; Final 3-5 days after receipt | Reference Range: By report |
| Limitations: | Interpretation: |
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Specimen Requirements |
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| Specimen Collection: Dependent upon type of specimen to be tested. | Sample Type: Pure culture |
| Volume/Amount Required: 1 slant or agar plate | Preferred Specimen: Pure Culture safely contained |
| Collection/Preservation: Ambient temperature | Storage Instructions: Store at temperature and atmospheric conditions that are suitable for growth dependent on organism. |
| Causes for Rejection: Insufficient amount of sample to test; no identifying markers on sample and/or paperwork. | Sample Container: Agar slant, handled as infectious agent. |
| Sample Test Kit: | Availability: Tested Monday-Friday |
| Diagnostic Information: Prior notification is requested. (512) 776-3781. Bioterrorism Investigation Section. CDC serology available only with prior approval and with submission of patient history. Reports of confirmed Bacillus anthracis will be called to the submitter immediately upon completion of testing. Please insure that an accurate telephone number and contact name is included on the submission form. For suspected Bioterrorism agents, see Bioterrorism agents (Referred -identification) For PCR testing see: Bioterrorism agents (Referred Identification - PCR). | |
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Specimen Submission |
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| Required Request Form: G-27A | Specimen Handling: Handled as infectious agent using universal precautions. |
| Transport Temperature: Ambient temperature | Shipping Requirements: Triple contained in accordance with federal shipping regulations for infectious agents. |
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Billing |
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| CPT Code: 87073, 87075 | Fees: |
Microbiology
Anthrax (Serological)
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| Test Includes: EIA for total anthrax antibodies | |
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Reporting |
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| Results Available: 3 weeks | Contact #s:512-458-7760 |
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Reference |
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| Method: EIA | |
| Turnaround Time: 3 weeks | Reference Range: <1.00 |
| Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. | Interpretation: Nonreactive |
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Specimen Requirements |
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| Specimen Collection: Venipuncture | Sample Type: Serum |
| Volume/Amount Required: 10 mL whole blood | Preferred Specimen: Serum, Paried Serum |
| Collection/Preservation: Red top or tiger top tube | Storage Instructions: Do not freeze whole blood. Separate serum from the clot if possible store in a screw cap vial |
| Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing, hemolysis | Sample Container: red top or tiger top vacutainer |
| Sample Test Kit: | Availability: Specimen will be sent to the CDC in Atlanta |
| Diagnostic Information: Prior notification is requested, 512-458-7760. A single serum specimen is acceptable, however, in order to determine if it is a recent infection, an acute and convalescent specimen drawn at least 14-16 days apart is necessary. A detailed patient history is required. Forwarded by TDSHS to CDC for testing. | |
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Specimen Submission |
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| Required Request Form: G-2B | Specimen Handling: Use Universal Precautions |
| Transport Temperature: Ambient temperature for specimens on the blood clot, separated serum at 2-8°C (refrigerated) or -20°C (frozen). | Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice. |
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Billing |
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| CPT Code: 87001 | Fees: |
Microbiology
Arizona Salmonella (Arizona) Culture (Identification)
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| Test Includes: Conventional Biochemical studies, Serological typing, Pulse-Field Gel Electrophoresis | |
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Reporting |
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| Results Available: 7-14 days | Contact #s: (512) 458-7784 |
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Reference |
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| Method: API 20E, Conventional biochemicals, Serological typing “O” , “H” , and Vi determination, Pulse-Field Gel Electrophoresis | |
| Turnaround Time: 7-14 days | Reference Range: By Report |
| Limitations: Organism must be viable and in pure culture. | Interpretation: Determination of the “O” and “H” antigens allows for complete serological typing of the organism. Pulse-Field Gel Electrophoresis is performed to determine relatedness to other strains identified. |
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Specimen Requirements |
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| Specimen Collection: | Sample Type: Pure culture |
| Volume/Amount Required: One specimen per patient | Preferred Specimen: Pure culture on agar slant |
| Collection/Preservation: 24 hour growth on agar slant | Storage Instructions: Ambient temperature |
| Causes for Rejection: Broken specimen | Sample Container: Agar slant in test tube. |
| Sample Test Kit: | Availability: Test performed Monday-Friday |
| Diagnostic Information: Organisms will be identified as Salmonella species. See Salmonellosis Culture –(Typing) | |
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Specimen Submission |
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| Required Request Form: G-2B | Specimen Handling: Infectious agents handled using universal precautions. |
| Transport Temperature: Ambient temperature | Shipping Requirements: Triple contained in accordance with federal shipping regulations for infectious agents. |
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Billing |
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| CPT Code: 87045 | Fees: |
Microbiology
Ascariasis (Microscopic - Direct Concentration)
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| Test Includes: Microscopic examination, direct concentration | |
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Reporting |
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| Results Available: 1 day | Contact #s: (512) 458-7760 |
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Reference |
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| Method: Microscopic examination, direct concentration | |
| Turnaround Time: 24 hours | Reference Range: No parasites found |
| Limitations: Parasites or ova must be visible under microscopic examination. | Interpretation: No parasites found indicates that there were no visually detectable parasites in the specimen. |
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Specimen Requirements |
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| Specimen Collection: Fecal specimens must be sent in as fresh (less that five hours) or in formalin. | Sample Type: Feces; Worm; Sputum; Tissue Section |
| Volume/Amount Required: stool 10 g | Preferred Specimen: Feces; Worm; Sputum; Tissue Section |
| Collection/Preservation: Fecal specimens must be sent in as fresh (less that five hours) or in formalin. | Storage Instructions: Ambient temperature |
| Causes for Rejection: Specimen not preserved and too old. | Sample Container: Clean, dry container, tightly sealed. |
| Sample Test Kit: | Availability: Monday-Friday |
| Diagnostic Information: Detection of eggs in fecal specimens must be sent in as fresh (less that five hours) or in formalin. Adult worms should be submitted in ethyl alcohol or formalin. Referred material accepted from hospital, private, and reference labs. | |
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Specimen Submission |
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| Required Request Form: G-2B | Specimen Handling: Body fluids handled with universal precautions. Formalin is a poison, handle with caution. |
| Transport Temperature: Ambient temperature | Shipping Requirements: Triple contained in accordance with federal shipping regulations for diagnostic specimens. |
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Billing |
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| CPT Code: 87177 | Fees: |