MosquitoID

Mosquito identification – Surveillance
Laboratory Fee Schedule
Procedure #: MZZ0411A
CPT: N/A

Synonym(s):	Mosquito ID and mosquito testing
Requisition Form	G-14
Test Description	Mosquito identification and pooling followed by homogenization of the adult mosquitoes in bovine albumin diluent to prepare the mosquito samples for RNA extraction and cell culture inoculation for the detection of arboviruses.
Pre-Approval Needed	Mosquitoes are accepted only from agencies that have been approved for participation in the Texas Department of State Health Services surveillance program.
Supplemental Information Required	N/A
Supplemental Form(s)	N/A
Performed on Specimens from (sources)	Environmental
Sample/Specimen Type for Testing	Live, adult mosquitoes
Minimum Volume/Size Required	N/A
Storage/Preservation Prior to Shipping	Mosquitoes must be kept cool, not frozen prior to shipping and should be shipped as soon as possible after collection, preferably on the same day as collection.
Transport Medium	N/A
Specimen Labeling	Each mosquito carton must have its own G-14 submission form completely filled out and attached using a rubber band.
Shipping and Specimen Handling Requirements	Ship in insulated boxes with coolant bottles or polar packs wrapped with moistened newspaper. Ship Monday through Thursday only. Do NOT ship on Friday.
Method	Taxonomic identification via microscopy Real-time RT-PCR Cell culture
Turn-around Time	21 days.
Interferences/Limitations	Mosquitoes should be shipped as quickly as possible once collected. Pesticides will contaminate collection equipment and shipment boxes. All participants in the program are advised to ship live mosquitoes.
Common Causes for Rejection	Failure to meet submission/shipping guidelines.
Additional Information	N/A