PCR: Burkholderia mallei/pseudomallei

Laboratory Fee Schedule 

Procedure #:  BTA0026
CPT:  87798

Synonym(s):  Melioidosis or Burkholderia pseudomallei, glanders or Burkholderia mallei PCR
Requisition Form G-27A
Test Description Detection of Burkholderia mallei/pseudomallei DNA by PCR
Pre-Approval Needed Must notify BioThreat Team prior to sample submission: (512) 689-5537
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources)
  • Non-Human (Bacterial)
  • Human
  • Environmental
Sample/Specimen Type for Testing
  • Culture isolates
  • Whole blood
  • Serum
  • Environmental specimens
Minimum Volume/Size Required Culture isolates
  • 1 agar slant or plate
Clinical samples
  • 1 mL
Storage/Preservation Prior to Shipping Culture isolates 
  • Ambient
Clinical or environmental samples 
  • 2-8°C (cold) 
Transport Medium N/A
Specimen Labeling Human
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match submission form.
Non-Human or Environmental
  • Sample identification must match submission form.
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
  • Handled as infectious agent using universal precautions. 
  • Triple contained in accordance with federal shipping regulations for infectious agents. 
  • See also Emergency Preparedness resources online.

Notify BioThreat Team prior to sample submission: (512) 689-5537

Method PCR
Turn-around Time Preliminary result 1 day after receipt, final result 1- 21 days
Interferences/Limitations Improper storage or shipping temperatures may limit organism recovery.
Common Causes for Rejection •    Insufficient quantity
•    Unacceptable specimen type or source
•    Improper shipping conditions
•    Expired media or collection container
•    Discrepancies between specimen label and submission form
•    Incomplete or missing submission form
Additional Information May be paired with Definitive ID: Brucella species