Mycobacterium tuberculosis complex, AFB Agar Proportion Drugs, Ofloxacin Susceptibility

Laboratory Fee Schedule
Procedure #: MAD0063A
CPT: 87190

Synonym(s):	M. tuberculosis complex drug susceptibility test (DST), OFLOX DST, OFL DST, OFX DST
Requisition Form	G-MYCO
Test Description	Susceptibility test to Ofloxacin by agar proportion method.
Pre-Approval Needed	N/A
Pre-Approval Needed	N/A
Supplemental Information Required	N/A
Performed on Specimens from (sources)	Pure isolate from human sources.
Sample/Specimen Type for Testing	M. tuberculosis complex pure isolate in liquid or on solid culture medium.
Minimum Volume/Size Required	Liquid culture - 2.0 mL Solid culture - Visible growth, more than a single colony
Storage/Preservation Prior to Shipping	Ambient
Transport Medium	N/A
Specimen Labeling	Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number) Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements	Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49 Triple-contain in accordance with federal shipping regulations for UN2814 Category A, Infectious substance, affecting humans. Anyone who handles Category A substances is required by law to be trained in handling and shipping Category A, Infectious Substances. Untrained personnel should not handle or ship M. tuberculosis isolates. Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
Method	Agar proportion susceptibility test interpreted as either susceptible or resistant to a specific drug at critical concentration recommended by CLSI.
Turn-around Time	14 to 21 days.
Interferences/Limitations	Test can only be performed on pure cultures of M. tuberculosis complex.
Common Causes for Rejection	Non-viable isolates. Cultures overgrown by contaminating organisms. Isolate not M. tuberculosis complex.
Additional Information	Isolates submitted for this test will be identified by this laboratory. No fee will be charged for this identification unless identification is requested on the G-MYCO requisition form. This laboratory will notify submitter of new drug resistance detected by this test.