Neisseria gonorrhoeae Antimicrobial Susceptibility (GC AST)

Laboratory Fee Schedule
Procedure #:  N/A

Synonym(s): GC AST
Requisition Form G-2B
Test Description Antimicrobial susceptibility testing by ETest gradient strip for Azithromycin, Cefixime, Ceftriaxone, and Ciprofloxacin for suspected drug resistant Neisseria gonorrhoeae.
Pre-Approval Needed NA
Supplemental Information Required Provide summary of treatment/testing. 
Provide most recent international or interstate travel within 1 month prior to specimen collection.  Attach to submission form or write on reverse side of submission form. 
Supplemental Form(s) NA
Performed on Specimens from (sources) urethra, throat, oropharynx, rectum,
vagina, cervix, conjunctiva
Sample/Specimen Type for Testing
  • Pure Isolate
  • Clinical Sample
Minimum Volume/Size Required
  • Pure Isolate: At least one viable colony
  • Clinical: At least one viable colony on transport media such as Copan ESwab
Storage/Preservation Prior to Shipping
  • Pure Isolate:  should be grown on a non-selective medium (Chocolate II or GC base + 1% growth supplements) and incubated for 16-18 hours at 35-37°C in a 4-6% CO2-enriched atmosphere. 
  • Pure isolate alternative: Bacterial culture should be resuspended (≥4 McFarland) in trypticase soy broth (TSB) with 15-20% glycerol and freeze immediately at -70°C. 
  • Clinical sample: ESwabs: store in transport media at room temperature.
Transport Medium Copan ESwab Transport for clinical samples available from DSHS upon request. 
Acceptable transport medium: Copan ESwab,
InTray GC, Jembec plates, Transgrow bottle. 
Specimen Labeling
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, medical record number)
  • Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
  • Ship isolate growth and clinical specimens overnight at ambient temperature Monday-Thursday to avoid weekend deliveries.  
  • Ship specimens in trypticase soy broth with glycerol which are frozen at -70°C on dry ice overnight  Monday-Thursday to avoid weekend deliveries.  Dry ice shippers and dry ice are not provided by DSHS. 
Method ETest Gradient strip AST
Turn-around Time 10 days
  • Specimen must be viable for testing. 
Common Causes for Rejection
  • Insufficient quantity
  • Unacceptable specimen type or source
  • Improper shipping conditions
  • Expired media or collection container
  • Discrepancies between specimen label and submission form
  • Incomplete or missing submission form
  • Specimens not suspected of treatment failure
Additional Information
  • To order the test choose “Neisseria” and write “GC AST test”
  • Identification confirmation and beta-lactamase testing will also be performed.