Nitrate + Nitrite – Drinking Water

Laboratory Fee Schedule
Group: Inorganic
Procedure #:  EZZ0047A

Synonym(s):  Nitrate and nitrite as nitrogen in potable water
Requisition Form G-22
Test Description The determination of nitrite singly, or nitrate and nitrite combined in drinking water samples. 
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Environmental
Sample/Specimen Type for Testing Drinking water
Minimum Volume/Size Required 100 mL
Storage/Preservation Prior to Shipping
  • Store in hard or soft plastic or glass container.
  • Measure and record the chlorine residual.
  • Maintain sample at 4.0°C.
  • Some headspace is allowed.
Transport Medium N/A
Specimen Labeling Sample identification must match submission form.
Shipping and Specimen Handling Requirements
  • Samples without chlorine residuals must be tested by laboratory within 48 hours of collection.
  • Samples submitted for nitrite analysis must be tested by laboratory within 48 hours of collection. 
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
Method Automated colorimetry (EPA method 353.2) 
Interferences/Limitations Some known interferences are as follows: 
  • residual chlorine. 
  • high concentrations of iron, copper, or other metals; 
  • high concentrations of oil or grease; and 
  • excessive turbidity. 
Common Causes for Rejection
  • Insufficient quantity
  • unacceptable specimen type or source
  • improper shipping conditions
  • expired media or collection container
  • discrepancies between specimen label and submission form
  • incomplete or missing submission form
Additional Information N/A