Laboratory Fee Schedule
Procedure: N/A
CPT: N/A
| Synonym(s): | Rabies PCR, Lyssavirus |
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| Requisition Form | G-9 |
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| Test Description | Testing of brain tissue for viable rabies virus by real-time PCR. |
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| Pre-Approval Needed | N/A |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Deceased Non-Human Mammals |
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| Sample/Specimen Type for Testing | Brain tissue from: - All large animal specimens negative for rabies virus by DFA testing.
- Specimens with inconclusive or unsatisfactory results by DFA testing.
- Rabies DFA weak positive specimens.
- All “decomposed if negative” and “destroyed if negative” specimens resulted as “decomposed” or “destroyed by rabies DFA testing.
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| Minimum Volume/Size Required | - Animal head with intact brain (whole carcasses of small mammals such as bats, rats, or mice are accepted).
- Fresh brain tissue: complete transverse cross section of brain stem and a cross section of cerebellum containing all 3 lobes.
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| Storage/Preservation Prior to Shipping | - Store at refrigeration temperatures (2°C–8°C).
- Do not submit live animals.
- Do not preserve tissue in formalin.
- Do not freeze.
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| Transport Medium | N/A |
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| Specimen Labeling | Specimen labels and identification must match submission form. |
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| Shipping and Specimen Handling Requirements | |
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| Method | Real-time PCR |
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| Turn-around Time | 16 days |
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| Interferences/Limitations | Decomposition or destruction of brain tissue. |
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| Common Causes for Rejection | - Insufficient specimen submitted (minimum volume not met).
- Improperly stored specimen (extreme heat, fixed in formalin).
- Failure to meet shipping or submission guidelines.
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| Additional Information | - This is a confirmatory test only and cannot be requested by submitters.
- Laboratory will determine testing need.
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