Shigella spp. – Identification

Laboratory Fee Schedule
Procedure #:  MAQ0113A 
CPT:  N/A
 

Synonym(s): Shigellosis
Requisition Form G-22-(Food); G-23
Test Description BAX® PCR Screen and conventional biochemical test and enrichment to isolate and identify Shigella species
Pre-Approval Needed DSHS Regulatory Agency OR Emerging and Acute Infectious Disease Unit Agency
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Food
Sample/Specimen Type for Testing
  • Food/milk samples
  • Environmental Swabs
Minimum Volume/Size Required 100 grams (¼ pound)
Storage/Preservation Prior to Shipping

Food

  • Place food sample in sterile zip-lock or whirlpack bag or other sterile plastic container. 
  • Store on ice.

Environmental swabs

  • Environmental swabs must be collected on appropriate swabs used for environmental testing (e.g., 3M™ Quick Swabs or Spongesicle type swabs or equivalent). 
Transport Medium N/A
Specimen Labeling Sample Identification must match submission form.
Shipping and Specimen Handling Requirements
  • Ship samples on Ice and/or Ice Packs.
  • Samples must be shipped for overnight delivery.
  • Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
Method
  • BAX® Real-Time PCR Screen
  • FDA Bacteriological Analytical Manual (BAM)- Chapter 6
Turn-around Time 14 Days
Interferences/Limitations The sample should represent the outer surface as well as the interior of the food. 
Common Causes for Rejection
  • Insufficient specimen
  • Not collected by a Registered Sanitarian
  • Improperly stored.
  • Failure to meet shipping guidelines.
Additional Information N/A