Candida spp. Isolate Testing

Laboratory Fee Schedule
Procedure Number: N/A

Synonym(s):	*• Candidiasis, C. auris, multidrug resistant yeast, antifungal susceptibility test, MALDI-TOF*
Requisition Form	• G-2E
Test Description	• Definitive identification of Candida isolates by MALDI-TOF, antifungal susceptibility determined by broth micro dilution and E-Test.
Pre-Approval Needed	• N/A
Supplemental Information Required	• Copies of any previous Lab tests (MALDI identification), if available.
Supplemental Form(s)	• N/A
Performed on Specimens from (sources)	• N/A
Sample/Specimen Type for Testing	• Pure culture of Candida isolate from any type of clinical specimen • Isolates should be submitted on media that supports the growth of yeast, such as Sabouraud Dextrose agar or Chromagar. • All Candida species except for confirmed C. albicans
Minimum Volume/Size Required	• At least one viable pure isolate per plate/slant
Storage/Preservation Prior to Shipping	• Isolates may be shipped to Laboratory at ambient temperatures. • Ensure that growth media used does not expire before use.
Transport Medium	• N/A
Specimen Labeling	• At least two patient identifiers required on specimen (e.g., patient full name, date of birth, medical record number). • Three patient identifiers are preferred. • Patient identifiers on specimen label must exactly match identifiers in submission form. • Additional DSHS Laboratory specimen labeling guidelines available online here.
Shipping and Specimen Handling Requirements	• Ship at ambient temperatures. • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49 • Category B Biological Substance, UN3373 shipping requirements apply • Submitters are responsible for packaging and shipping specimens according to regulatory requirements. • Additional DSHS Laboratory submission guidelines available online here.
Method	• Identification of Candida by MALDI TOF. • Antifungal susceptibility testing by broth micro dilution and ETest.
Turn-around Time	• 10 working days
Interferences/Limitations	• These results should be used only for epidemiological purposes and not for patient treatment.
Common Causes for Rejection	• Mixed culture/contamination submitted. • Non-viable isolates. • Missing identifiers on specimen and/or submission form. • Media expired at time of isolate culture • Mismatch(es) between patient identifiers on G-2E submission form and specimen label.
Additional Information	N/A