Candida spp. Isolate Testing

Laboratory Fee Schedule
Procedure Number: N/A 
 

Synonym(s): 
  • Candidiasis, C. auris, Candidozyma, multidrug resistant yeast, antifungal susceptibility test, MALDI-TOF
Requisition Form
  • G-2E
Test Description
  • Definitive identification of Candida isolates by MALDI-TOF, antifungal susceptibility determined by broth micro dilution and E-Test.
Pre-Approval Needed
  • N/A
Supplemental Information Required
  • Copies of any previous Lab tests (MALDI identification), if available.
Supplemental Form(s)
  • N/A
Performed on Specimens from (sources)
  • Human
Sample/Specimen Type for Testing
  • Pure culture of Candida isolate from any type of clinical specimen.
  • Isolates should be submitted on media that supports the growth of yeast, such as Sabouraud Dextrose agar or Chromagar.
  • All Candida species except for confirmed C. albicans.
Minimum Volume/Size Required
  • At least one viable pure isolate per plate/slant
Storage/Preservation Prior to Shipping
  • Isolates may be shipped to Laboratory at ambient temperatures.
  • Ensure that growth media used does not expire before use. 
Transport Medium
  • N/A
Specimen Labeling
  • At least two patient identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
  • Three patient identifiers are preferred.  
  • Patient identifiers on specimen label must exactly match identifiers on submission form. (e.g., abbreviated name on label and full name on form is not acceptable.)
  • Additional DSHS Laboratory specimen labeling guidelines available online here.
Shipping and Specimen Handling Requirements
  • Ship at ambient temperatures.
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
  • Category B Biological Substance, UN3373 shipping requirements apply to unidentified isolates.
  • Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
  • Additional DSHS Laboratory specimen shipping guidelines online here.
  • Ship overnight on cold packs if specimens are refrigerated.
  • Ship specimens overnight Monday–Thursday to avoid weekend deliveries.
  • Do not ship specimens for holiday or weekend delivery.
  • Ensure media used to isolate specimens is not expired.
  • Additional DSHS Laboratory submission guidelines available online here.
Method
  • Identification of Candida by MALDI TOF.
  • Antifungal susceptibility testing by broth micro dilution and ETest.
Turn-around Time
  • 10 working days
Interferences/Limitations
  • These results should be used only for epidemiological purposes and not for patient treatment.
Common Causes for Rejection
  • Mixed culture/contaminated specimen submitted.
  • Non-viable isolates submitted.
  • Missing patient identifiers on specimen and/or submission form.
  • Media expired at time of isolate culturing.
  • Mismatch(es) between patient identifiers on G-2E submission form and specimen label.
Additional Information
  • N/A