Synonym(s): |
- Carbapenem Resistant Enterobacterales, Carbapenem Resistant Pseudomonas aeruginosa, Carbapenem Resistant Acinetobacter baumanii, CRE, CRPA, CRAB, CRO, ARLN, Carbapenem, Antibiotic resistance, Antibiotic Susceptibility Test, PCR, Antimicrobial, MALDI-TOF, mCIM, Broth Micro Dilution
|
---|
Requisition Form |
G-2E |
---|
Test Description |
- Definitive identification of CRO isolates by MALDI-TOF, antimicrobial susceptibility determined by broth micro dilution, and carbapenemase production determination by real-time PCR.
|
---|
Pre-Approval Needed |
|
---|
Supplemental Information Required |
- Antibiotic susceptibility report from submitter
- Copies of any previous Lab tests (carbapenemase production or PCR), if available.
|
---|
Supplemental Form(s) |
|
---|
Performed on Specimens from (sources) |
|
---|
Sample/Specimen Type for Testing |
- Pure cultured CRO isolate from any type of clinical specimen
- Isolates should be submitted on a non-inhibitory, non-selective agar plate or/slant or broth
- CRE isolates resistant to any imipenem, meropenem, doripenem or ertapenem
- CRPA isolates resistant to at least one imipenem, meropenem, or doripenem AND non-susceptible (intermediate or resistant) to cefepime or ceftazidime
- Acinetobater baumanii isolates resistant to imipenem, meropenem, or doripenem.
|
---|
Minimum Volume/Size Required |
- At least one viable pure isolate per plate/slant; tube of pure culture isolate
|
---|
Storage/Preservation Prior to Shipping |
- Isolates may be shipped to Laboratory at ambient temperatures
- Ensure that growth media used does not expire before specimen collection date
|
---|
Transport Medium |
|
---|
Specimen Labeling |
- At least two patient identifiers required on specimen (e.g., patient full name, date of birth, medical record number).
- Three patient identifiers are preferred.
- Identifiers on specimen must exactly match identifiers in submission form.
- Additional DSHS Laboratory specimen labeling guidelines available online here.
|
---|
Shipping and Specimen Handling Requirements |
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49
- Category B Biological Substance, UN3373 shipping requirements apply
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- Additional DSHS Laboratory submission guidelines available online here.
|
---|
Method |
- Identification of CRE/CRPA/CRAB by MALDI TOF.
- Antibiotic susceptibility testing by broth micro dilution.
- Carbapenemase production in CRE and CRPA determined by modified Carbapenem Inactivation Method (mCIM). Carbapenemase producing CRE/CRPA and CRAB identified by real-time PCR.
|
---|
Turn-around Time |
|
---|
Interferences/Limitations |
- These results should be used only for epidemiological purposes and not for patient treatment.
|
---|
Common Causes for Rejection |
- Mixed culture submitted
- Antibiotic susceptibility result from submitter not provided
- Missing identifiers on specimen and/or submission form
- Isolate sent not a target organism
- Media expired at time of isolate culture
- Mismatch between specimen and form identifiers
|
---|
Additional Information |
|
---|