Influenza SARS-CoV-2 (COVID-19) Multiplex Real-Time RT-PCR

Laboratory Fee Schedule
Procedure #:  MCN0312A

Synonym(s):  Influenza Surveillance, SARS-CoV-2, COVID-19
Requisition Form G-2V.
Test Description Testing of samples for the detection and differentiation of SARS-CoV-2, influenza A virus, and/or influenza B virus nucleic acid in upper and lower respiratory specimens from individuals suspected of respiratory viral infection consistent with COVID-19.
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N./A
Performed on Specimens from (sources) Human
Sample/Specimen Type for Testing
  • Preferred Specimen
    • Nasopharyngeal swabs
  • Alternative Specimens
    • Oropharyngeal (throat) swabs
    • Nasal swabs
    • Sputum
    • Lower respiratory tract aspirates
    • Bronchoalveolar lavage
    • Nasopharyngeal wash/aspirate
    • Nasal aspirate
Minimum Volume/Size Required 2° – 3 mL.
Storage/Preservation Prior to Shipping
  • Store at 2° – 8°C for up to 72 hours
  • Store frozen, at -70°C or below, for storage longer than 72 hours cold. 
Transport Medium
  • Universal transport media (UTM)
  • Viral transport media (VTM)
Specimen Labeling
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number).
  • Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements Ship samples: 
  • According to Dangerous Good Regulations, IATA, and/or CFR 49.
  • Handling as infectious agent using universal precautions.
  • Triple-contained in accordance with federal shipping regulations for infectious agents.
  • If specimens are expected to arrive at the lab within 72 hours of collection, refrigerate at 2-8°C and ship overnight on ice packs.
  • If specimens are expected to arrive at the lab after 72 hours of collection, freeze at -70°C and ship overnight on dry ice.
Method Real-Time RT-PCR
Turn-around Time 2 business days.
  • False negative may result if specimen is improperly collected, transported, or handled.
  • Detection of viral RNA does not imply that the corresponding virus is infectious or is the causative agent for clinical symptoms.
  • A negative result does not preclude infection.
Common Causes for Rejection
  • Information on the submission form and sample do not match.
  • Failure to put two forms of identification on sample.
  • Specimen received cold greater than 72 hours after collection.
  • Specimen received ambient.
  • Specimen leaked in transit.
  • Submission of incorrect sample/specimen type.
  • Submission of incorrect or expired transport medium.
Additional Information Please mark the Influenza Surveillance {Influenza PCR} and COVID-19 (SARS-CoV-2) PCR boxes on the G-2V form.
Note: By checking the Influenza Surveillance or COVID-19 PCR test request box, submitters authorize DSHS to test for Flu and/or COVID as resources allow.