| Synonym(s): | Arbovirus, Flavivirus, serology, ELISA |
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| Requisition Form | G-2V |
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| Test Description | IgM capture ELISA for the qualitative detection of IgM
antibodies to Dengue virus in serum for the presumptive
diagnosis of Dengue fever. |
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| Pre-Approval Needed | Contact your local or state epidemiologist if chikungunya,
dengue or Zika virus are suspected. Contact information can
be found at the following link
https://www.dshs.texas.gov/idps-investigationforms/ |
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| Supplemental Information Required | N/A |
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| Supplemental Form(s) | N/A |
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| Performed on Specimens from (sources) | Human |
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| Sample/Specimen Type for Testing | Serum collected in Serum Separator tubes (SST) or red top tubes. |
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| Minimum Volume/Size Required | 1.0 mL. |
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| Storage/Preservation Prior to Shipping | - Store serum at 2°C to 8°C for up to 48 hours after
collection. - For storage longer than 48 hours, store frozen at -20°C or
below. - Never freeze serum in serum separator (SST) tubes.
- Transfer serum to transport tube before freezing.
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| Transport Medium | N/A |
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| Specimen Labeling | - At least two patient-specific identifiers required on
specimen (e.g. patient full name, date of birth, medical
record number). - Three unique patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match
identifiers on G-2V submission form. (e.g. abbreviated
name on label and full name on form is not acceptable.) - Additional DSHS Laboratory specimen labeling guidelines
available online here.
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| Shipping and Specimen Handling Requirements | - Ship according to Dangerous Goods Regulations, IATA,
and/or CFR 49. - Category B Biological Substance, UN3373 shipping
requirements apply. - Submitters are responsible for packaging and shipping
specimens according to regulatory requirements. - Additional DSHS Laboratory specimen shipping guidelines
online here. - Store and ship specimens cold at 2°C –8°C and ship
overnight on cold packs if they will arrive at the
Laboratory within 48 hours of collection. - Store and ship specimens frozen at -20°C or colder and ship overnight on dry ice if specimens
will arrive at the lab more than 48 hours after time of collection, freeze. - Additional DSHS Laboratory specimen shipping guidelines
online here.
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| Method | Enzyme-Linked Immunosorbent Assay (ELISA) |
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| Turn-around Time | 7 working days |
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| Interferences/Limitations | - Cross-reactivity with other flaviviruses is very common.
Sera from patients infected with Japanese Encephalitis,
West Nile, and/or Saint Louis viruses may give false
positive results. - Repeated freezing and thawing of samples can cause
antibody deterioration.
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| Common Causes for Rejection | - insufficient specimen quantity
- unacceptable specimen type or source
- improper shipping conditions
- expired collection container
- discrepancies between specimen label and submission
form - incomplete, illegible, or missing submission form
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| Additional Information | - Reactive samples will be forwarded to CDC for
confirmatory testing by Plaque Reduction Neutralization
Test (PRNT). - Minimum volume for CDC confirmation testing 0.5 mL.
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