Synonym(s):
|
Ebola PCR
|
Requisition Form
|
G-27A
|
Test Description
|
Detection
of Ebola Zaire RNA by PCR
|
Pre-Approval
Needed
|
DSHS
Epidemiology consultation with physician. Must notify BioThreat Team
prior to sample submission: (512) 689-5537
|
Supplemental
Information Required
|
N/A
|
Supplemental
Form(s)
|
N/A
|
Performed on
Specimens from (sources)
|
Human
|
Sample/Specimen
Type for Testing
|
-
Whole
blood
- Serum
- Plasma
- Urine (with paired blood sample only)
|
Minimum
Volume/Size Required
|
1 ml for fluids
|
Storage/Preservation
Prior to Shipping
|
Clinical
samples - 2-8°C (cold)
- can be stored up to 7 days
Serum and plasma - may be frozen at <-70°C if a delay is anticipated
|
Transport Medium
|
Purple top EDTA tubes for blood
|
Specimen Labeling
|
- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form.
|
Shipping and
Specimen Handling Requirements
|
- Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
- Handled as infectious agent using universal precautions.
- Triple contained in accordance with federal shipping regulations for
infectious agents.
- See also Emergency Preparedness resources online.
- Notify BioThreat Team prior to sample submission:
(512) 689-5537
|
Method
|
PCR
|
Turn-around Time
|
1 day
after receipt
|
Interferences/Limitations
|
Improper storage or shipping temperatures may limit organism recovery.
|
Common Causes for
Rejection
|
-
Insufficient quantity
- unacceptable
specimen type or source
- improper shipping conditions
- expired media or
collection container
- discrepancies between specimen label and submission
form
- incomplete or missing submission form
|
Additional
Information
|
N/A
|