Hepatitis B Surface Antigen


Hepatitis B Surface Antigen

Laboratory Fee Schedule

Procedure: MBK0213A

CPT: 87340

Hepatitis B Surface Antigen
Synonym(s): Enzyme Immunoassay (EIA) for the Detection of Hepatitis B Surface Antigen (HBsAg) in Human Serum Specimens 
Requisition Form G-2A 
Test DescriptionA qualitative enzyme immunoassay for the detection of Hepatitis B Surface Antigen (HBsAg) in human serum 
Pre-Approval NeededN/A 
Supplemental Information Required N/A
Supplemental Form(s) N/A 
Performed on Specimens from (sources) Human 
Sample/Specimen Type for Testing Serum 
Minimum Volume/Size Required500 µL 
Storage/Preservation Prior to ShippingSerum separated from the clot may be stored at 2°C to 4°C up to 48 hours, but then must be frozen at -20°C or colder 
Transport MediumN/A 
Specimen Labeling At least 2 patient specific identifiers: First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. Medical Record number); must match information on submission form (G-2A). 
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
  • Handled as infectious agent using universal precautions. 
  • Triple contained in accordance with federal shipping regulations for infectious agents. 
    Additional forms & resources
MethodEIA for the detection of antibodies 
Turn-around Time4 working days 
Interferences/Limitations A person who has been vaccinated for hepatitis B may have transient reactivity for up to 18 days.
Common Causes for Rejection Insufficient quantity, unacceptable specimen type or source, improper shipping conditions, expired media or collection container, discrepancies between specimen label and submission form, and/or incomplete or missing submission form 
Additional Information A designation of reactive for HBsAg must not be based on a single reactive test result.   
Last updated April 13, 2022